Long non-coding RNA expression profiles in gallbladder carcinoma identified using microarray analysis
文献类型：期刊论文
| 作者 | Wang, Jiwen1,2; Liu, Han2; Shen, Xiaokun5; Wang, Yueqi2; Zhang, Dexiang4; Shen, Sheng2; Suo, Tao2; Pan, Hongtao2; Ming, Yue3; Ding, Kan1
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| 刊名 | ONCOLOGY LETTERS
 |
| 出版日期 | 2017-05 |
| 卷号 | 13期号:5页码:3508-3516 |
| 关键词 | computational biology expression profile gallbladder neoplasms long non-coding RNA gene microarray analysis |
| ISSN号 | 1792-1074 |
| DOI | 10.3892/ol.2017.5893 |
| 文献子类 | Article |
| 英文摘要 | Gallbladder carcinoma (GBC) is the most common biliary tract cancer and exhibits poor patient prognosis. Previous studies have identified that long non-coding RNAs (lncRNAs) serve important regulatory roles in cancer biology. Alterations in lncRNAs are associated with several types of cancer. However, the contribution of IncRNAs to GBC remains unclear. To investigate the lncRNAs that are potentially involved in GBC, IncRNA profiles were identified in three pairs of human GBC and corresponding peri-carcinomatous tissue samples using microarray analysis. Reverse transcription -quantitative polymerase chain reaction (RT-qPCR) was used to validate the microarray data. in order to elucidate potential functions, Gene Ontology, Kyoto Encyclopedia of Genes and Genomes analysis, and network analysis were used to determine relevant signaling pathways. Abundant RNA probes were used, and 1,758 IneRNAs and 1,254 mRNAs were detected to he differentially expressed by the microarray. Compared with para-carcinoma tissue, numerous IncRNAs were markedly upregulated or downregulated in GBC. The results demonstrated that the IncRNAs that were downregulated in GBC were more numerous compared with the IncRNAs that were upregulated. Among them, RP11I-152P172-006 was the most upregulated, whereas CTA-941F9.9 was the most downregulated. The RT-qPCR results were consistent with the microarray data. Pathway analysis indicated that live pathways corresponded to the differentially expressed transcripts. It was demonstrated that IncRNA expression in GBC was markedly altered, and a series of novel IneRNAs associated with GBC were identified. The results of the present study suggest that the functions of lncRNAs are important in GBC development and progression. |
| WOS关键词 | CANCER-CELLS ; HOTAIR ; METASTASIS ; PROLIFERATION ; MANAGEMENT ; PROMOTES ; DISEASE ; MYC |
| 资助项目 | National Natural Science Foundation of China[81272728] |
| WOS研究方向 | Oncology |
| 语种 | 英语 |
| WOS记录号 | WOS:000401129800091 |
| 出版者 | SPANDIDOS PUBL LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272686]  |
| 专题 | 药理学第三研究室 |
| 通讯作者 | Ding, Kan; Liu, Houbao |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Glycobiol & Glycochem Lab, 555 Zu Chongzhi Rd, Shanghai 201203, Peoples R China; 2.Fudan Univ, Gen Surg Inst, Zhongshan Hosp, Dept Gen Surg, 180 Fenglin Rd, Shanghai 200032, Peoples R China; 3.Chinese Acad Med Sci, Peking Union Med Coll, Canc Hosp, PET CT Ctr, Beijing 100021, Peoples R China 4.Fudan Univ, Peoples Hosp Shanghai 5, Dept Gen Surg, Shanghai 200240, Peoples R China; 5.Wenzhou Med Univ, Taizhou Hosp, Dept Surg Oncol, Taizhou 317000, Zhejiang, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Wang, Jiwen,Liu, Han,Shen, Xiaokun,et al. Long non-coding RNA expression profiles in gallbladder carcinoma identified using microarray analysis[J]. ONCOLOGY LETTERS,2017,13(5):3508-3516. |
| APA | Wang, Jiwen.,Liu, Han.,Shen, Xiaokun.,Wang, Yueqi.,Zhang, Dexiang.,...&Liu, Houbao.(2017).Long non-coding RNA expression profiles in gallbladder carcinoma identified using microarray analysis.ONCOLOGY LETTERS,13(5),3508-3516. |
| MLA | Wang, Jiwen,et al."Long non-coding RNA expression profiles in gallbladder carcinoma identified using microarray analysis".ONCOLOGY LETTERS 13.5(2017):3508-3516. |
入库方式： OAI收割
来源：上海药物研究所
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