Isolation and Structural Characterization of the Polysaccharides of Cortex Mori radices
文献类型：期刊论文
| 作者 | Zhang Xiaoman; Liao Wenfeng; Cong Qifei; Dong Qun; Ding Kan
|
| 刊名 | ACTA CHIMICA SINICA
 |
| 出版日期 | 2013-05-15 |
| 卷号 | 71期号:5页码:722-728 |
| 关键词 | cortex mori radices Morus alba L. polysaccharide chemical structure antitumor |
| ISSN号 | 0567-7351 |
| DOI | 10.6023/A13010109 |
| 文献子类 | Article |
| 英文摘要 | Cortex mori radices, the dried root cortex of Morus alba L., is a clinically widely used traditional Chinese medicine, with the effects of anti-tussive, preventing asthma, hypoglycemic, antitumor, antibacterial and antioxidative. Up to now, its polysaccharides and their contribution to biological activities remain largely unknown. In this study, the root cortex was extracted with boiling water, followed by ethanol precipitation, fractionation and purification by anion-exchange chromatography on DEAE-cellulose column and gel permeation chromatography on Sephacryl S-300 column. Three polysaccharides, CMA-a-1, CMA-a-5 and CMA-bl-1, were isolated from boiling-water extract, and characterized to be homogeneous fractions by high performance gel permeation chromatography (BPGPC). Their structural features were elucidated by sugar analyses, methylation analyses, IR, and NMR spectroscopic methods. Both CMA-a-1 and CMA-a-5 are found to be starch fractions. CMA-a-1, with an average molecular weight (MW) of 224 kDa, is an amylopectin-like polysaccharide with an average chain length (CL) of 23, containing 1,4-linked alpha-D-glucopyranosyl backbone, with 1, 6-linked glucose in the side chains attached to O-6 of backbone residues. CMA-a-5, with a MW of 15.5 kDa, is mainly an amylose fraction with an average chain length of 30. CMA-bl-1 was isolated from 0.2 mol.L-1 NaCl-eluted fraction upon anion-exchange chromatography, and characterized as a RG-I type pectic polysaccharide, which contains a backbone composed of alternating 1,4-linked alpha-D-galacturonic acid and 1,2-linked alpha-L-rhamnose residues, with various branches attached to 0-4 of rhamnose residues, comprising of T-, 1,3-, 1,5-, 1,3,5-linked Araf, T-, 1,6-, 1,3,6-linked Galp, 1, 4-linked Xylp, and 1, 4-linked Glcp. MTT method showed that CMA-bl-1 could inhibit the proliferation of SMMC7721 liver cancer cells and showed no impairment to L02 normal liver cells. This study suggested that the boiling water extract of cortex mori radices contains mainly starch and pectic fraction as the major component polysaccharides, and that the RG-I polysaccharide in vitro can inhibit the liver cancer cell proliferation, but the starch fractions cannot. |
| 资助项目 | National Natural Science Foundation of China[30870551] ; Key New Drug Creation and Manufacturing Program[2012ZX09301001-003] |
| WOS研究方向 | Chemistry |
| 语种 | 中文 |
| CSCD记录号 | CSCD:4836158 |
| WOS记录号 | WOS:000320011200005 |
| 出版者 | SCIENCE PRESS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277617]  |
| 专题 | 药理学第三研究室 |
| 通讯作者 | Zhang Xiaoman |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang Xiaoman,Liao Wenfeng,Cong Qifei,et al. Isolation and Structural Characterization of the Polysaccharides of Cortex Mori radices[J]. ACTA CHIMICA SINICA,2013,71(5):722-728. |
| APA | Zhang Xiaoman,Liao Wenfeng,Cong Qifei,Dong Qun,&Ding Kan.(2013).Isolation and Structural Characterization of the Polysaccharides of Cortex Mori radices.ACTA CHIMICA SINICA,71(5),722-728. |
| MLA | Zhang Xiaoman,et al."Isolation and Structural Characterization of the Polysaccharides of Cortex Mori radices".ACTA CHIMICA SINICA 71.5(2013):722-728. |
入库方式： OAI收割
来源：上海药物研究所
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