Production and Antitumor Bioactivity of rAPC(Recombinant Allophycocyanin)Expressed in a Recombinant E. coli Strain
文献类型：期刊论文
| 作者 | Tang Zhihong1; Qin Song1; Wu Shaojie1; Gao Jiangtao1; Lin Liping2; Ding Jian2
|
| 刊名 | High Technology Letters
 |
| 出版日期 | 2004 |
| 卷号 | 14期号:3页码:83-86 |
| 关键词 | Recombinant allophycocyanin Recombinant E. coli High cell density culture High expression Antitumor activity |
| ISSN号 | 1002-0470 |
| 其他题名 | 镭普克的制备及对小鼠H22肝癌的抑制作用 |
| 文献子类 | Article |
| 英文摘要 | High cell density fermentation of a rAPC producing E. coli strain at 20L scale, purification and antitumor effects of rAPC were studied. One-step and two-step fermentations were employed and the result showed that both expression effi-ciency and biomass of rAPC in two-step fennentation was much higher than those in one-step fermentation. After affinity and gel filtration chromatography, the purity of rAPC reached 98.03% . Inhibitory rate of rAPC on H22 hepatomae could reach 62.2% at the dose of 50 mg/kg per day by i.v application. It was also revealed by experiments that rAPC could inhance the function of B cells by i. p. Result of this paper suggests a promising prospect of large-scale production of rAPC and potential clinical application. |
| 资助项目 | 863计划[00000000] ; 中国科学院创新工程[00000000] |
| WOS研究方向 | Engineering (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:1606331 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/268421]  |
| 专题 | 科研与新药推进处 药理学第一研究室 |
| 作者单位 | 1.Institute of Oceanology, Chinese Academy of Sciences, Qingdao, Shandong 266071, China.; 2.Shanghai Institute of Drug, Chinese Academy of Sciences, Shanghai 200031, China. |
| 推荐引用方式 GB/T 7714 | Tang Zhihong,Qin Song,Wu Shaojie,et al. Production and Antitumor Bioactivity of rAPC(Recombinant Allophycocyanin)Expressed in a Recombinant E. coli Strain[J]. High Technology Letters,2004,14(3):83-86. |
| APA | Tang Zhihong,Qin Song,Wu Shaojie,Gao Jiangtao,Lin Liping,&Ding Jian.(2004).Production and Antitumor Bioactivity of rAPC(Recombinant Allophycocyanin)Expressed in a Recombinant E. coli Strain.High Technology Letters,14(3),83-86. |
| MLA | Tang Zhihong,et al."Production and Antitumor Bioactivity of rAPC(Recombinant Allophycocyanin)Expressed in a Recombinant E. coli Strain".High Technology Letters 14.3(2004):83-86. |
入库方式： OAI收割
来源：上海药物研究所
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