Effects of stable isotope labeled internal standard on determination of ivabradine and N-demethylivabradine in human plasma
文献类型：期刊论文
| 作者 | Liu Dongqin2; Yu Jinghua2; Zhang Yifan2 ; Zhong Dafang2; He Ling1; Chen Xiaoyan2
|
| 刊名 | Acta Pharmaceutica Sinica
 |
| 出版日期 | 2015 |
| 卷号 | 50期号:3页码:348-354 |
| 关键词 | LC-MS/MS stable isotope labeled internal standard LC-MS/MS ivabradine N-demethylivabradine |
| ISSN号 | 0513-4870 |
| 其他题名 | 稳定同位素内标对人血浆样品中伊伐布雷定和 N-去甲基伊伐布雷定测定的影响 |
| 文献子类 | Article |
| 英文摘要 | This study aims to develop a liquid chromatography with tandem mass spectrometry (LC-MS/MS) method for the simultaneous determination of ivabradine and N-demethylivabradine in human plasma, and investigate effects of stable isotope labeled (SIL) internal standard (IS) on ivabradine. The analytes and IS were extracted from plasma by protein precipitation with acetonitrile, and chromatographied on a Capcell PAK C18 (100 mm * 4.6 mm, 5 mum) column using a mobile phase of methanol and 5 mmol·L~(-1) ammonium acetate. Multiple reaction monitoring with electrospray ionization (ESI) was used in the positive mode for mass spectrometric detection. The effect of ivabradine isotope peak [M+H+3]~+ on IS and the effect of SIL IS purity on ivabradine were evaluated. An appropriate concentration of SIL IS was chosen to permit method selectivity and linearity of the assay over the required range. The standard curves were demonstrated to be linear in the range of 0.100 to 60.0 ng·mL~(-1) for ivabradine, and 0.050 0 to 20.0 ng·mL~(-1) for N-demethylivabradine. The intra and inter day precision and accuracy were within the acceptable limits for all concentrations. Besides, the interaction between IS and ivabradine did not impact the determination of analytes. This method was successfully applied to a pharmacokinetic study of hydrogen sulfate ivabradine sustained release tablets on Chinese healthy volunteers. |
| WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:5360090 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/269294]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Chen Xiaoyan |
| 作者单位 | 1.College of Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu 210009, China. 2.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.; |
| 推荐引用方式 GB/T 7714 | Liu Dongqin,Yu Jinghua,Zhang Yifan,et al. Effects of stable isotope labeled internal standard on determination of ivabradine and N-demethylivabradine in human plasma[J]. Acta Pharmaceutica Sinica,2015,50(3):348-354. |
| APA | Liu Dongqin,Yu Jinghua,Zhang Yifan,Zhong Dafang,He Ling,&Chen Xiaoyan.(2015).Effects of stable isotope labeled internal standard on determination of ivabradine and N-demethylivabradine in human plasma.Acta Pharmaceutica Sinica,50(3),348-354. |
| MLA | Liu Dongqin,et al."Effects of stable isotope labeled internal standard on determination of ivabradine and N-demethylivabradine in human plasma".Acta Pharmaceutica Sinica 50.3(2015):348-354. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。