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PDE5 inhibitor promotes melanin synthesis through the PKG pathway in B16 melanoma cells

文献类型：期刊论文

作者Zhang, Xiaodong1; Yan, Guirui1; Ji, Jun1,2; Wu, Jingwei1; Sun, Xiaoyun1; Shen, Jingshan1; Jiang, Hualiang1; Wang, Heyao1
刊名JOURNAL OF CELLULAR BIOCHEMISTRY
出版日期2012-08
卷号113期号:8页码:2738-2743
关键词MELANIN SYNTHESIS PDE5 INHIBITOR PKG TYROSINASE
ISSN号0730-2312
DOI10.1002/jcb.24147
文献子类Article
英文摘要PDE inhibitors could increase cellular cGMP levels and are used to treat erectile dysfunction as well as pulmonary arterial hypertension. cGMP production was reported to be necessary for UVB-induced melanin synthesis, however, the effect of PDE5 inhibitor on melanin synthesis has not been examined. We found that PDE5 inhibitor (sildenafil or vardenafil) and the cGMP analog 8-CPT-cGMP stimulated CREB phosphorylation, leading to increased tyrosinase expression and melanin synthesis, which was counteracted by KT5823, a selective cGMP-dependent protein kinase (PKG) inhibitor. However, KT5823 did not affect cAMP-elevating agent-mediated melanin synthesis, indicating that KT5823 selectively inhibited cGMP-induced melanin synthesis. This is the first study to find that PDE5 inhibitor can promote melanin synthesis and reveal that PKG-dependent CREB phosphorylation and tyrosinase expression is involved in cGMP-induced melanin synthesis. Our results suggest that PDE5 inhibitor may be beneficial for the treatment of hypopigmentation diseases. J. Cell. Biochem. 113: 27382743, 2012. (c) 2012 Wiley Periodicals, Inc.
WOS关键词TRANSCRIPTION FACTOR ; HUMAN MELANOCYTES ; KINASE ACTIVATION ; NITRIC-OXIDE ; MELANOGENESIS ; SKIN ; CREB ; PIGMENTATION ; DISORDERS ; NETWORK
资助项目Shanghai Science and Technology Innovation Program[08431900800] ; National Natural Science Foundation of China[81072681] ; National Natural Science Foundation of China[20972174] ; National Science and Technology Major[2009ZX09301-001] ; National Science and Technology Major Project "Key New Drug Creation and Manufacturing Program"[2009ZX09301-001]
WOS研究方向Biochemistry & Molecular Biology ; Cell Biology
语种英语
WOS记录号WOS:000305334900021
出版者WILEY-BLACKWELL
源URL[http://119.78.100.183/handle/2S10ELR8/278007]  
专题药理学第三研究室
通讯作者Wang, Heyao
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China;
2.Fudan Univ, Sch Pharm, Shanghai 200032, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Xiaodong,Yan, Guirui,Ji, Jun,et al. PDE5 inhibitor promotes melanin synthesis through the PKG pathway in B16 melanoma cells[J]. JOURNAL OF CELLULAR BIOCHEMISTRY,2012,113(8):2738-2743.
APA Zhang, Xiaodong.,Yan, Guirui.,Ji, Jun.,Wu, Jingwei.,Sun, Xiaoyun.,...&Wang, Heyao.(2012).PDE5 inhibitor promotes melanin synthesis through the PKG pathway in B16 melanoma cells.JOURNAL OF CELLULAR BIOCHEMISTRY,113(8),2738-2743.
MLA Zhang, Xiaodong,et al."PDE5 inhibitor promotes melanin synthesis through the PKG pathway in B16 melanoma cells".JOURNAL OF CELLULAR BIOCHEMISTRY 113.8(2012):2738-2743.

入库方式： OAI收割

来源：上海药物研究所

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