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Sildenafil promotes adipogenesis through a PKG pathway

文献类型：期刊论文

作者Zhang, Xiaodong1; Ji, Jun1,2; Yan, Guirui1; Wu, Jingwei1; Sun, Xiaoyun1; Shen, Jingshan1; Jiang, Hualiang1; Wang, Heyao1
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
出版日期2010-06-11
卷号396期号:4页码:1054-1059
关键词Adipogenesis PDE5 inhibitor PKG Adipocytes
ISSN号0006-291X
DOI10.1016/j.bbrc.2010.05.064
文献子类Article
英文摘要Sildenafil is the first oral PDE5 inhibitor for the treatment of erectile dysfunction and pulmonary arterial hypertension. In the present study, we investigated the effect of sildenafil on adipogenesis in 3T3L1 pre-adipocytes. Treatment with sildenafil for 8 days significantly promoted adipogenesis characterized by increased lipid droplet and triglyceride content in 3T3L1 cells. Meanwhile, sildenafil induced a pronounced up-regulation of the expression of adipocyte-specific genes, such as aP2 and GLUT4. The results by RT-PCR and Western blotting further showed that sildenafil increased the sequential expression of C/EBP beta, PPAR gamma and C/E8P alpha. Additionally, we found that the other two PDE5 inhibitors (vardenafil and tadalafil) and the cGMP analog 8-pCPT-cGMP also increased adipogenesis. Likewise, 8-pCPT-cGMP could upregulate the expression of adipogenic and adipocyte-specific genes. Importantly, the PKG inhibitor Rp-8pCPT-cGMP was able to inhibit both sildenafil and 8-pCPT-cGMP-induced adipogenesis. Furthermore, sildenafil promoted basal and insulin-mediated glucose uptake in 3T3L1 cells, which was counteracted by Rp-8-pCPT-cGMP. These results indicate that sildenafil could promote adipogenesis accompanied by increased glucose uptake through a PKG pathway at least partly. (C) 2010 Elsevier Inc. All rights reserved.
WOS关键词NITRIC-OXIDE ; PPAR-GAMMA ; C/EBP-ALPHA ; ADIPOCYTE DIFFERENTIATION ; ADIPOSE-TISSUE ; PULMONARY-HYPERTENSION ; GUANYLYL CYCLASE ; CGMP ; ACTIVATION ; CITRATE
资助项目National High Technology Research and Development Program of China (863 Program)[2007AA02Z301] ; National Science and Technology Major Project[2009ZX09301-001] ; Shanghai Science and Technology Innovation Program[08431900800] ; State Key Program of Basic Research of China[2009CB918502]
WOS研究方向Biochemistry & Molecular Biology ; Biophysics
语种英语
WOS记录号WOS:000279125100047
出版者ACADEMIC PRESS INC ELSEVIER SCIENCE
源URL[http://119.78.100.183/handle/2S10ELR8/278874]  
专题药理学第三研究室
通讯作者Wang, Heyao
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China;
2.Fudan Univ, Sch Pharm, Shanghai 200032, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Xiaodong,Ji, Jun,Yan, Guirui,et al. Sildenafil promotes adipogenesis through a PKG pathway[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2010,396(4):1054-1059.
APA Zhang, Xiaodong.,Ji, Jun.,Yan, Guirui.,Wu, Jingwei.,Sun, Xiaoyun.,...&Wang, Heyao.(2010).Sildenafil promotes adipogenesis through a PKG pathway.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,396(4),1054-1059.
MLA Zhang, Xiaodong,et al."Sildenafil promotes adipogenesis through a PKG pathway".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 396.4(2010):1054-1059.

入库方式： OAI收割

来源：上海药物研究所

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