A structurally novel salt-regulated promoter of duplicated carbonic anhydrase gene 1 from Dunaliella salina
文献类型：期刊论文
| 作者 | Li, Jie1; Lu, Yumin1; Xue, Lexun1; Xie, Hua2
|
| 刊名 | MOLECULAR BIOLOGY REPORTS
 |
| 出版日期 | 2010-02 |
| 卷号 | 37期号:2页码:1143-1154 |
| 关键词 | bar gene Dunaliella salina Duplicated carbonic anhydrase 1 Promoter |
| ISSN号 | 0301-4851 |
| DOI | 10.1007/s11033-009-9901-z |
| 文献子类 | Article |
| 英文摘要 | It has been demonstrated that the duplicated carbonic anhydrase is induced by salt in the Dunaliella salina (D. salina) and duplicated carbonic anhydrase 1 (DCA1) is a member of carbonic anhydrase family. The purpose of this study was to identify whether both the DCA1 gene and its promoter from D. salina are salt-inducible. In this study, the results of real time RT-PCR showed that the transcripts of DCA1 were induced by gradient concentration of sodium chloride. Subsequently, a structurally novel promoter containing highly repeated GT/AC sequences of the DCA1 gene was isolated, which was able to drive a stable expression of the foreign bar gene in transformed cells of D. salina, and the gradient concentrations of sodium chloride in media paralleled regulations in the levels of both proteins and mRNA of the bar gene driven by the DCA1 promoter. Furthermore, analysis of GUS activities revealed that the salt-inducible expression of the external gus gene was regulated by the promoter fragments containing highly repeated GT sequences, but not by the promoter fragments deleting highly repeated GT sequences. The findings above-mentioned suggest that the highly repeated GT sequence in the DCA1 promoter is involved in the salt-inducible regulation in D. salina and may be a novel salt-inducible element. |
| WOS关键词 | NITRATE REDUCTASE GENE ; ALGA DUNALIELLA ; FOREIGN GENES ; EXPRESSION ; PROTEIN ; HALOTOLERANCE ; SYSTEM |
| 资助项目 | Ministry of Science and Technology of P. R. China[2007DFA01240] ; National Natural Science Foundation of China[30700014] ; Medical science and technique Foundation of Henan province of China[200803021] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000273085800063 |
| 出版者 | SPRINGER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278985]  |
| 专题 | 药理学第一研究室 |
| 通讯作者 | Xue, Lexun |
| 作者单位 | 1.Zhengzhou Univ, Dept Biol, Cell Biol Lab, Zhengzhou 450001, Henan, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Jie,Lu, Yumin,Xue, Lexun,et al. A structurally novel salt-regulated promoter of duplicated carbonic anhydrase gene 1 from Dunaliella salina[J]. MOLECULAR BIOLOGY REPORTS,2010,37(2):1143-1154. |
| APA | Li, Jie,Lu, Yumin,Xue, Lexun,&Xie, Hua.(2010).A structurally novel salt-regulated promoter of duplicated carbonic anhydrase gene 1 from Dunaliella salina.MOLECULAR BIOLOGY REPORTS,37(2),1143-1154. |
| MLA | Li, Jie,et al."A structurally novel salt-regulated promoter of duplicated carbonic anhydrase gene 1 from Dunaliella salina".MOLECULAR BIOLOGY REPORTS 37.2(2010):1143-1154. |
入库方式： OAI收割
来源：上海药物研究所
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