STUDIES ON ANTITUMOR ACTIVITY OF A WATER-SOLUBLE HYPERBRANCHED POLYSACCRARIDE AND ITS SULFATED DERIVATIVES
文献类型：期刊论文
| 作者 | Tao Yongzhen3,4; Zhang Lina3; Han Lu1; Zeng Fanbo1; Ding Kan2
|
| 刊名 | ACTA POLYMERICA SINICA
 |
| 出版日期 | 2009-09-20 |
| 期号 | 9页码:852-859 |
| 关键词 | Hyperbranched polysaccharide Molecular size Chain conformation Sulfation Antitumor activity |
| ISSN号 | 1000-3304 |
| 文献子类 | Article |
| 英文摘要 | A water-soluble hyperbranchend beta-glucan, extracted from sclerotia of Pleurotas tuber-regium was fractionated to obtain the fractions with different molecular weight. The polysaccharide fractions were treated with chlorosulfonic acid to synthesize sulfated derivatives. The antitumor activities of the water-soluble hyperbranched polysaccharide fractions and their sulfated derivatives were evaluated by in vivo test using SARCOMA 180 xenografted BALB/C mice,and in vitro measurement against HepG2 cell, respectively. The effect of introduction of sulfate groups in the polysaccharide on antitumor activities was compared with that of the native polysaccharides by morphological, histological and ELISA methods. The results indicated that the native polysaccharides,in the radius of gyration(< S(2)>(1/2)(z)) range from 42.5 nor to 11 3 nm, exhibited significantly inhibition impact on the xenografted tumor cell growth and antiproliferative activities against HepG2 cell in vitro. The antitumor activities of the sulfated derivatives increased progressively with the < S(2)>(1/2)(z) change from 17.8 nm to 34.9 nm. Mechanism study indicated that the native and sulfated glucans inhibited the proliferation of HepG2 cell through inducing the tumor cells apoptosis in a time dependent way. Immunological test demonstrated that both the native polysasaccharide and its sulfated derivatives using low dose (20 mg/kg) increased production of tumor necrosis factor alpha ( TNF-alpha) in mouse plasma while the native one decreased amount of TNF-alpha using high does (60 mg/kg) with or without liopolysaccharide (LPS) stimulation. |
| WOS关键词 | PHYSICOCHEMICAL PROPERTIES ; GANODERMA-LUCIDUM ; POLYSACCHARIDE ; APOPTOSIS ; NECROSIS ; GLUCANS ; SCLEROTIUM ; AGENT ; MICE |
| WOS研究方向 | Polymer Science |
| 语种 | 中文 |
| CSCD记录号 | CSCD:3684516 |
| WOS记录号 | WOS:000270319800002 |
| 出版者 | SCIENCE PRESS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279125]  |
| 专题 | 药理学第三研究室 |
| 通讯作者 | Tao Yongzhen |
| 作者单位 | 1.Huazhong Univ Sci & Technol, Tongji Med Coll, Wuhan 430030, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Glycochem & Glycobiol Lab, Shanghai 201203, Peoples R China 3.Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China; 4.Wuhan Univ Sci & Engn, Minist Educ, Key Lab Green Proc & Funct Text New Textile Mat, Wuhan 430073, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Tao Yongzhen,Zhang Lina,Han Lu,et al. STUDIES ON ANTITUMOR ACTIVITY OF A WATER-SOLUBLE HYPERBRANCHED POLYSACCRARIDE AND ITS SULFATED DERIVATIVES[J]. ACTA POLYMERICA SINICA,2009(9):852-859. |
| APA | Tao Yongzhen,Zhang Lina,Han Lu,Zeng Fanbo,&Ding Kan.(2009).STUDIES ON ANTITUMOR ACTIVITY OF A WATER-SOLUBLE HYPERBRANCHED POLYSACCRARIDE AND ITS SULFATED DERIVATIVES.ACTA POLYMERICA SINICA(9),852-859. |
| MLA | Tao Yongzhen,et al."STUDIES ON ANTITUMOR ACTIVITY OF A WATER-SOLUBLE HYPERBRANCHED POLYSACCRARIDE AND ITS SULFATED DERIVATIVES".ACTA POLYMERICA SINICA .9(2009):852-859. |
入库方式： OAI收割
来源：上海药物研究所
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