Enantioselective determination of 3-n-butylphthalide (NBP) in human plasma by liquid chromatography on a teicoplanin-based chiral column coupled with tandem mass spectrometry
文献类型：期刊论文
| 作者 | Diao, Xingxing; Ma, Zhiyu; Lei, Peng; Zhong, Dafang ; Zhang, Yifan; Chen, Xiaoyan
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| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2013-11-15 |
| 卷号 | 939页码:67-72 |
| 关键词 | 3-n-Butylphthalide (NBP) Teicoplanin-based chiral column LC-MS/MS Enantioselective pharmacokinetics |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2013.09.014 |
| 文献子类 | Article |
| 英文摘要 | A novel and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated to determine the exposure of 3-n-butylphthalide (NBP) enantiomers in human plasma. The NBP enantiomers were extracted from human plasma using methyl tert-butyl ether. The baseline separation of R-(+)-NBP and S-(-)-NBP was achieved within 11.0 min using a teicoplanin-based Astec Chirobiotic T column (250 mm x 4.6 mm i.d., 5 mu m) under isocratic conditions at a flow rate of 0.6 mL/min. The selection of the chiral stationary phase and the effect of the mobile phase composition on the resolution of the enantiomers were discussed. The selectivity, linearity, precision, accuracy, matrix effect, recovery, and stability were evaluated under optimized conditions. The LC-MS/MS method using 200 mu L of human plasma was linear over the concentration range of 5.00-400 ng/mL for each enantiomer. The lower limit of quantification (LLOQ) for both enantiomers was 5.00 ng/mL. The intra- and inter-assay precision values of the replicated quality control samples were within 8.0% for each enantiomer. The mean accuracy values for the quality control samples were within +/- 6.1% of the nominal values for R-(+)-NBP and S-(-)-NBP. No chiral inversion was observed during sample storage, preparation, and analysis. The method proved suitable for enantioselective pharmacokinetic studies of NBP after an oral administration of a therapeutic dose of racemic NBP. (C) 2013 Elsevier B.V. All rights reserved. |
| WOS关键词 | IMPROVES COGNITIVE IMPAIRMENT ; CEREBRAL-BLOOD-FLOW ; MAJOR METABOLITES ; L-3-N-BUTYLPHTHALIDE ; RATS ; DL-3-N-BUTYLPHTHALIDE ; BETA |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000326010400010 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277374]  |
| 专题 | 上海药物代谢研究中心 |
| 通讯作者 | Chen, Xiaoyan |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Diao, Xingxing,Ma, Zhiyu,Lei, Peng,et al. Enantioselective determination of 3-n-butylphthalide (NBP) in human plasma by liquid chromatography on a teicoplanin-based chiral column coupled with tandem mass spectrometry[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2013,939:67-72. |
| APA | Diao, Xingxing,Ma, Zhiyu,Lei, Peng,Zhong, Dafang,Zhang, Yifan,&Chen, Xiaoyan.(2013).Enantioselective determination of 3-n-butylphthalide (NBP) in human plasma by liquid chromatography on a teicoplanin-based chiral column coupled with tandem mass spectrometry.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,939,67-72. |
| MLA | Diao, Xingxing,et al."Enantioselective determination of 3-n-butylphthalide (NBP) in human plasma by liquid chromatography on a teicoplanin-based chiral column coupled with tandem mass spectrometry".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 939(2013):67-72. |
入库方式： OAI收割
来源：上海药物研究所
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