Optimization, characterization, sulfation and antitumor activity of neutral polysaccharides from the fruit of Borojoa sorbilis cuter
文献类型：期刊论文
| 作者 | Xu, Fangfang3; Liao, Kangsheng3; Wu, Yunshan3; Pan, Qi3; Wu, Lilan3; Jiao, Hong2; Guo, Dean1,3 ; Li, Ben3; Liu, Bo3
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| 刊名 | CARBOHYDRATE POLYMERS
 |
| 出版日期 | 2016-10-20 |
| 卷号 | 151页码:364-372 |
| 关键词 | Borojoa sorbilis cuter Polysaccharide Structural characterization Sulfation Antitumor activity |
| ISSN号 | 0144-8617 |
| DOI | 10.1016/j.carbpol.2016.05.091 |
| 文献子类 | Article |
| 英文摘要 | Extraction optimization, purification, characterization, sulfation and antitumor activity of polysaccharides from the fruit body of Borojoa sorbilis cuter were investigated in present study. The optimal Ultrahigh Pressure extraction condition was determined as: extraction once with the solid-liquid ratio of 1:10 in 30 degrees C and 1500 Mpa for crude polysaccharide (BP) and experimental yield was 8.28%. Four water-soluble polysaccharides named as BP1-1, BP1-2, BP1-3 and BP1-4, with molecular weight of 35.8, 32.4, 30.1 and 27.7 kDa, were purified by DEAE Sepharose and Superdex 200 chromatography. On the basis of chemical and spectroscopic analyses, BP1-1-BP1-4 were found to be neutral beta-D-galactan containing a (1 -> 4) linked backbone. S-BP1s with the DSS of 1.18, was sulfated by chloro-sulfonic acid-pyridine method. Furthermore, S-BP1s exhibited significant in vitro antitumor activity against liver cancer HepG2 and lung cancer A549 cells in a dose-dependent manner. The results indicated that S-BP1s could be potentially developed as functional antitumor drug. (C) 2016 Elsevier Ltd. All rights reserved. |
| WOS关键词 | STRUCTURAL-CHARACTERIZATION ; ANTICOAGULANT ACTIVITY ; BIOLOGICAL-ACTIVITIES ; GANODERMA-ATRUM ; GREEN-ALGA ; ANTIOXIDANT ; DERIVATIVES ; EXTRACTION |
| 资助项目 | National Natural Science Foundation of China[81202398] ; Guangdong provincial science and technology project[2013B010102006] ; Guangdong Provincial Science and Technology Project[2014A020221035] ; Guangdong Provincial Science and Technology Project[2015A020211025] ; Specific Research Project of Science and Technology of Guangdong Provincial Hospital of TCM[YN2014ZHR209] ; Specific Research Project of Science and Technology of Guangdong Provincial Hospital of TCM[YN2015MS03] |
| WOS研究方向 | Chemistry ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000380943900042 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/275853]  |
| 专题 | 上海中药现代化研究中心 |
| 通讯作者 | Guo, Dean; Li, Ben; Liu, Bo |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat Technol, Shanghai Res Ctr Modernizat Tradit Chinese Med, 501 Haike Rd, Shanghai 201203, Peoples R China 2.Guangdong Entry Exit Inspect & Quarantine Bur, Guangzhou 510006, Guangdong, Peoples R China; 3.Guangzhou Univ Chinese Med, Guangdong Prov Acad Chinese Med Sci, Affiliated Hosp 2, Guangzhou 510006, Guangdong, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Xu, Fangfang,Liao, Kangsheng,Wu, Yunshan,et al. Optimization, characterization, sulfation and antitumor activity of neutral polysaccharides from the fruit of Borojoa sorbilis cuter[J]. CARBOHYDRATE POLYMERS,2016,151:364-372. |
| APA | Xu, Fangfang.,Liao, Kangsheng.,Wu, Yunshan.,Pan, Qi.,Wu, Lilan.,...&Liu, Bo.(2016).Optimization, characterization, sulfation and antitumor activity of neutral polysaccharides from the fruit of Borojoa sorbilis cuter.CARBOHYDRATE POLYMERS,151,364-372. |
| MLA | Xu, Fangfang,et al."Optimization, characterization, sulfation and antitumor activity of neutral polysaccharides from the fruit of Borojoa sorbilis cuter".CARBOHYDRATE POLYMERS 151(2016):364-372. |
入库方式： OAI收割
来源：上海药物研究所
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