Structure determination and activity manipulation of the turfgrass ABA receptor FePYR1
文献类型：期刊论文
| 作者 | Ren, Zhizhong7,8,9; Wang, Zhen7,8,9 ; Zhou, X. Edward1,6; Shi, Huazhong5; Hong, Yechun7,8,9; Cao, Minjie8,9; Chan, Zhulong4; Liu, Xue8,9; Xu, H. Eric1,3,6; Zhu, Jian-Kang2,8,9
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| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2017-10-25 |
| 卷号 | 7 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/s41598-017-14101-9 |
| 文献子类 | Article |
| 英文摘要 | Turfgrass are widely cultivated ornamental plants that have important ecological, societal and economical values. However, many turfgrass species are susceptible to drought and demand frequent irrigation thus consuming large amounts of water. With the ultimate goal of improving drought resistance in turfgrass, we identified several ABA receptors in turfgrass that are important to mediate ABA signaling and drought stress response. The ABA receptor FePYR1 from turfgrass Festuca elata was demonstrated to bind ABA as a monomer. Crystal structure analysis revealed that FePYR1 recognizes and binds ABA by the common gate-latch-lock mechanism resembling the Arabidopsis ABA receptors, but the ABA binding pocket in FePYR1 shows discrepant residues resulting in different binding affinity to ABA. Structure-guided alterations of amino acid residues in FePYR1 generated ABA receptor variants with significantly increased ABA binding affinity. Expression of FePYR1 in Arabidopsis conferred enhanced drought resistance in the transgenic plants. These findings provided detailed information about FePYR1 and demonstrated that structure-assisted engineering could create superior ABA receptors for improving plant drought resistance. The detailed structural information of FePYR1 would also assist future rational design of small molecules targeting specific ABA receptors in economically important plant species. |
| WOS关键词 | ABSCISIC-ACID RECEPTORS ; PROMOTES DROUGHT RESISTANCE ; SIGNAL-TRANSDUCTION ; MOLECULAR-BASIS ; PYL PROTEINS ; WATER-USE ; MECHANISM ; ACTIVATION ; BINDING ; STRESS |
| 资助项目 | Chinese Academy of Sciences[00000000] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000413700200061 |
| 出版者 | NATURE PUBLISHING GROUP |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/272441]  |
| 专题 | 药物靶标结构与功能中心 |
| 通讯作者 | Xu, H. Eric; Zhu, Jian-Kang |
| 作者单位 | 1.Van Andel Res Inst, Lab Struct Sci, Grand Rapids, MI USA; 2.Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47907 USA 3.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Struct & Funct Drug Targets, Key Lab Receptor Res,VARI SIMM Ctr, Shanghai, Peoples R China; 4.Huazhong Agr Univ, Coll Hort & Forest Sci, Minist Educ, Key Lab Hort Plant Biol, Wuhan 430070, Hubei, Peoples R China; 5.Texas Tech Univ, Dept Chem & Biochem, Lubbock, TX 79409 USA; 6.Van Andel Res Inst, Lab Struct Biol & Biochem, Grand Rapids, MI USA; 7.Univ Chinese Acad Sci CAS, Shanghai 200032, Peoples R China; 8.Chinese Acad Sci, Ctr Excellence Mol Plant Sci, Shanghai 200032, Peoples R China; 9.Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, Shanghai 200032, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Ren, Zhizhong,Wang, Zhen,Zhou, X. Edward,et al. Structure determination and activity manipulation of the turfgrass ABA receptor FePYR1[J]. SCIENTIFIC REPORTS,2017,7. |
| APA | Ren, Zhizhong.,Wang, Zhen.,Zhou, X. Edward.,Shi, Huazhong.,Hong, Yechun.,...&Zhu, Jian-Kang.(2017).Structure determination and activity manipulation of the turfgrass ABA receptor FePYR1.SCIENTIFIC REPORTS,7. |
| MLA | Ren, Zhizhong,et al."Structure determination and activity manipulation of the turfgrass ABA receptor FePYR1".SCIENTIFIC REPORTS 7(2017). |
入库方式： OAI收割
来源：上海药物研究所
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