Structural insights into alternative splicing-mediated desensitization of jasmonate signaling
文献类型：期刊论文
| 作者 | Zhang, Feng4,5,6,7,8; Ke, Jiyuan4,5; Zhang, Li6,9; Chen, Rongzhi6; Sugimoto, Koichi6; Howe, Gregg A.1,6,10; Xu, H. Eric2,5 ; Zhou, Mingguo7; He, Sheng Yang1,3,6,9; Melcher, Karsten4
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| 刊名 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
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| 出版日期 | 2017-02-14 |
| 卷号 | 114期号:7页码:1720-1725 |
| 关键词 | signaling plant hormone plant defense plant pathogen plant insect |
| ISSN号 | 0027-8424 |
| DOI | 10.1073/pnas.1616938114 |
| 文献子类 | Article |
| 英文摘要 | Jasmonate ZIM-domain (JAZ) transcriptional repressors play a key role in regulating jasmonate (JA) signaling in plants. Below a threshold concentration of jasmonoyl isoleucine (JA-Ile), the active form of JA, the C-terminal Jas motif of JAZ proteins binds MYC transcription factors to repress JA signaling. With increasing JA-Ile concentration, the Jas motif binds to JA-Ile and the COI1 subunit of the SCFCOI1 E3 ligase, which mediates ubiquitination and proteasomal degradation of JAZ repressors, resulting in derepression of MYC transcription factors. JA signaling subsequently becomes desensitized, in part by feedback induction of JAZ splice variants that lack the C-terminal Jasmotif but include an N-terminal crypticMYC-interaction domain (CMID). The CMID sequence is dissimilar to the Jas motif and is incapable of recruiting SCFCOI1, allowing CMID-containing JAZ splice variants to accumulate in the presence of JA and to re-repress MYC transcription factors as an integral part of reestablishing signal homeostasis. The mechanism by which the CMID represses MYC transcription factors remains elusive. Here we describe the crystal structure of the MYC3-CMIDJAZ10 complex. In contrast to the Jas motif, which forms a single continuous helix when bound to MYC3, the CMID adopts a loop-helix-loop-helix architecture with modular interactions with both the Jas-binding groove and the backside of the Jasinteraction domain of MYC3. This clamp-like interaction allows the CMID to bind MYC3 tightly and block access of MED25 (a subunit of the Mediator coactivator complex) to the MYC3 transcriptional activation domain, shedding light on the enigmatic mechanism by which JAZ splice variants desensitize JA signaling. |
| WOS关键词 | ARABIDOPSIS JAZ PROTEINS ; TRANSCRIPTION FACTORS ; GENE-EXPRESSION ; REPRESSION ; MOTIF ; CORONATINE ; RESPONSES ; RECEPTOR ; TARGETS ; ACID |
| 资助项目 | Michigan Technology Tri-Corridor[085P1000817] ; Office of Science of the US Department of Energy (DOE)[DE-AC02-06CH11357] ; China Scholarship Council[201206850026] ; Gordon and Betty Moore Foundation Grant[GBMF3037] ; NIH[R01AI060761] ; NIH[R01GM102545] ; NIH[GM104212] ; NIH[DK071662] ; DOE Chemical Sciences, Geosciences, and Biosciences Division, Office of Basic Energy Sciences, Office of Science[DE-FG02-91ER20021] ; Van Andel Research Institute[00000000] ; National Natural Science Foundation of China[31300245] ; Chinese Ministry of Science and Technology[2012ZX09301001] ; Chinese Ministry of Science and Technology[2012CB910403] ; Chinese Ministry of Science and Technology[2013CB910600] ; Chinese Ministry of Science and Technology[XDB08020303] ; Chinese Ministry of Science and Technology[2013ZX09507001] ; Amway-China[00000000] ; Japan Society for Promotion of Science Research Fellowship for Young Scientists[24-824] ; Michigan AgBioResearch[MICL02278] ; Michigan State University[00000000] ; Michigan Economic Development Corporation[00000000] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000393989300084 |
| 出版者 | NATL ACAD SCIENCES |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/275632]  |
| 专题 | 药物靶标结构与功能中心 |
| 通讯作者 | Zhou, Mingguo; He, Sheng Yang; Melcher, Karsten |
| 作者单位 | 1.Michigan State Univ, Plant Resilience Inst, E Lansing, MI 48824 USA; 2.Chinese Acad Sci, Key Lab Receptor Res, Shanghai Inst Mat Med, VARI SIMM Ctr,Ctr Struct & Funct Drug Targets,Sha, Shanghai, Peoples R China; 3.Michigan State Univ, Howard Hughes Med Inst, E Lansing, MI 48824 USA 4.Van Andel Res Inst, Lab Struct Biol & Biochem, Grand Rapids, MI 49503 USA; 5.Van Andel Res Inst, Lab Struct Sci, Grand Rapids, MI 49503 USA; 6.Michigan State Univ, Dept Energy, Plant Res Lab, E Lansing, MI 48824 USA; 7.Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China; 8.Wuhan Univ, Coll Life Sci, State Key Lab Hybrid Rice, Wuhan 430072, Peoples R China; 9.Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA; 10.Michigan State Univ, Dept Biochem & Mol Biol, E Lansing, MI 48824 USA; |
| 推荐引用方式 GB/T 7714 | Zhang, Feng,Ke, Jiyuan,Zhang, Li,et al. Structural insights into alternative splicing-mediated desensitization of jasmonate signaling[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2017,114(7):1720-1725. |
| APA | Zhang, Feng.,Ke, Jiyuan.,Zhang, Li.,Chen, Rongzhi.,Sugimoto, Koichi.,...&Melcher, Karsten.(2017).Structural insights into alternative splicing-mediated desensitization of jasmonate signaling.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,114(7),1720-1725. |
| MLA | Zhang, Feng,et al."Structural insights into alternative splicing-mediated desensitization of jasmonate signaling".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 114.7(2017):1720-1725. |
入库方式： OAI收割
来源：上海药物研究所
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