X-ray laser diffraction for structure determination of the rhodopsin-arrestin complex
文献类型:期刊论文
作者 | Zhou, X. Edward14; Gao, Xiang14; Barty, Anton15; Kang, Yanyong14; He, Yuanzheng14; Liu, Wei5,13; Ishchenko, Andrii4; White, Thomas A.15; Yefanov, Oleksandr15; Han, Gye Won3,4 |
刊名 | SCIENTIFIC DATA |
出版日期 | 2016-04-12 |
卷号 | 3 |
ISSN号 | 2052-4463 |
DOI | 10.1038/sdata.2016.21 |
文献子类 | Article; Data Paper |
英文摘要 | Serial femtosecond X-ray crystallography (SFX) using an X-ray free electron laser (XFEL) is a recent advancement in structural biology for solving crystal structures of challenging membrane proteins, including G-protein coupled receptors (GPCRs), which often only produce microcrystals. An XFEL delivers highly intense X-ray pulses of femtosecond duration short enough to enable the collection of single diffraction images before significant radiation damage to crystals sets in. Here we report the deposition of the XFEL data and provide further details on crystallization, XFEL data collection and analysis, structure determination, and the validation of the structural model. The rhodopsin-arrestin crystal structure solved with SFX represents the first near-atomic resolution structure of a GPCR-arrestin complex, provides structural insights into understanding of arrestin-mediated GPCR signaling, and demonstrates the great potential of this SFX-XFEL technology for accelerating crystal structure determination of challenging proteins and protein complexes. |
WOS关键词 | SERIAL FEMTOSECOND CRYSTALLOGRAPHY ; LIPIDIC CUBIC PHASE ; INDEXING AMBIGUITY ; CRYSTAL-STRUCTURE ; SPACE-GROUP ; INTEGRATION ; QUALITY |
资助项目 | Michigan Economic Development Corporation[00000000] ; Michigan Technology Tri-Corridor[085P1000817] ; Federal funds from the National Cancer Institute[ACB-12002] ; National Institute of General Medical Sciences[AGM-12006] ; Office of Science of the US Department of Energy[DE-AC02-06CH11357] ; Jay and Betty Van Andel Foundation[00000000] ; Ministry of Science and Technology (China)[2012ZX09301001] ; Ministry of Science and Technology (China)[2012CB910403] ; Ministry of Science and Technology (China)[2013CB910600] ; Ministry of Science and Technology (China)[XDB08020303] ; Ministry of Science and Technology (China)[2013ZX09507001] ; Amway (China)[00000000] ; National Institute of Health[DK071662] ; National Institute of Health[GM102545] ; National Institute of Health[GM104212] ; National Institutes of Health Common Fund in Structural Biology grants[P50 GM073197] ; National Institutes of Health Common Fund in Structural Biology grants[P50 GM073210] ; National Institutes of Health Common Fund in Structural Biology grants[R01 GM095583] ; National Institute of General Medical Sciences PSI: Biology grants[U54 GM094618] ; National Institute of General Medical Sciences PSI: Biology grants[R01 GM108635] ; National Institute of General Medical Sciences PSI: Biology grants[U54 GM094599] ; National Institute of General Medical Sciences PSI: Biology grants[GM097463] ; National Institute of General Medical Sciences PSI: Biology grants[U54 GM094586] ; NSF Science and Technology Center[1231306] ; Science Foundation Ireland[12/IA/1255] ; Helmholtz Gemeinschaft[00000000] ; DFG Cluster of Excellence Center for Ultrafast Imaging[00000000] ; BMBF project[FKZ 05K12CH1] |
WOS研究方向 | Science & Technology - Other Topics |
语种 | 英语 |
出版者 | NATURE PUBLISHING GROUP |
WOS记录号 | WOS:000390214400001 |
源URL | [http://119.78.100.183/handle/2S10ELR8/276078] |
专题 | 药物靶标结构与功能中心 |
通讯作者 | Zhou, X. Edward; Xu, H. Eric |
作者单位 | 1.Paul Scherrer Inst, Swiss Light Source, CH-5232 Villigen, Switzerland; 2.Trinity Coll Dublin, Sch Med, Dublin D02 R590, Ireland; 3.Univ Southern Calif, Bridge Inst, Dept Biol Sci, Los Angeles, CA 90089 USA; 4.Univ Southern Calif, Bridge Inst, Dept Chem, Los Angeles, CA 90089 USA; 5.Arizona State Univ, Ctr Appl Struct Discovery, Biodesign Inst, Tempe, AZ 85287 USA; 6.Ctr Ultrafast Imaging, D-22761 Hamburg, Germany; 7.Arizona State Univ, Dept Phys, Tempe, AZ 85287 USA; 8.SLAC Natl Accelerator Lab, Linac Coherent Light Source LCLS, Menlo Pk, CA 94025 USA; 9.SLAC Natl Accelerator Lab, Stanford Synchrotron Radiat Lightsource, Joint Ctr Struct Genom, Menlo Pk, CA 94025 USA; 10.Shanghai Tech Univ, IHuman Inst, 2F Bldg 6,99 Haike Rd, Shanghai 201210, Peoples R China; |
推荐引用方式 GB/T 7714 | Zhou, X. Edward,Gao, Xiang,Barty, Anton,et al. X-ray laser diffraction for structure determination of the rhodopsin-arrestin complex[J]. SCIENTIFIC DATA,2016,3. |
APA | Zhou, X. Edward.,Gao, Xiang.,Barty, Anton.,Kang, Yanyong.,He, Yuanzheng.,...&Xu, H. Eric.(2016).X-ray laser diffraction for structure determination of the rhodopsin-arrestin complex.SCIENTIFIC DATA,3. |
MLA | Zhou, X. Edward,et al."X-ray laser diffraction for structure determination of the rhodopsin-arrestin complex".SCIENTIFIC DATA 3(2016). |
入库方式: OAI收割
来源:上海药物研究所
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