High-resolution mass spectrometry of small molecules bound to membrane proteins
文献类型：期刊论文
| 作者 | Gault, Joseph3; Donlan, Joseph A. C.3; Liko, Idlir3; Hopper, Jonathan T. S.3; Gupta, Kallol3; Housden, Nicholas G.1; Struwe, Weston B.3; Marty, Michael T.3; Mize, Todd3; Bechara, Cherine4 |
| 刊名 | NATURE METHODS
 |
| 出版日期 | 2016-04 |
| 卷号 | 13期号:4页码:333-336 |
| ISSN号 | 1548-7091 |
| DOI | 10.1038/NMETH.3771 |
| 文献子类 | Article |
| 英文摘要 | Small molecules are known to stabilize membrane proteins and to modulate their function and oligomeric state, but such interactions are often hard to precisely define. Here we develop and apply a high-resolution, Orbitrap mass spectrometry-based method for analyzing intact membrane protein-ligand complexes. Using this platform, we resolve the complexity of multiple binding events, quantify small molecule binding and reveal selectivity for endogenous lipids that differ only in acyl chain length. |
| WOS关键词 | ESCHERICHIA-COLI ; GAS-PHASE ; LIPIDS ; ASSEMBLIES ; COMPLEXES ; BINDING |
| 资助项目 | Royal Commission for the Exhibition of 1851 Fellowship[00000000] ; Junior Research Fellowship at St Catherine's College, Oxford[00000000] ; Engineering and Physical Sciences Research Council[00000000] ; UK Medical Research Council[G1000819] ; European Research Council[268851] ; Royal Society Research Professorship[00000000] ; Wellcome Trust[104633/Z/14/Z] ; BBSRC[BB/L021234/1] ; Biotechnology and Biological Sciences Research Council[BB/L021234/1] ; Engineering and Physical Sciences Research Council[1240748] ; Medical Research Council[G1000819] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| 出版者 | NATURE PUBLISHING GROUP |
| WOS记录号 | WOS:000374084800020 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276090]  |
| 专题 | 药物靶标结构与功能中心 |
| 通讯作者 | Robinson, Carol V. |
| 作者单位 | 1.Univ Oxford, Dept Biochem, Oxford OX1 3QU, England; 2.Thermo Fisher Sci, Bremen, Germany; 3.Univ Oxford, Dept Chem, Phys & Theoret Chem Lab, Oxford OX1 3QZ, England; 4.Univ Montpellier, CNRS, UMR 5235, F-34059 Montpellier, France 5.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, Pudong, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Gault, Joseph,Donlan, Joseph A. C.,Liko, Idlir,et al. High-resolution mass spectrometry of small molecules bound to membrane proteins[J]. NATURE METHODS,2016,13(4):333-336. |
| APA | Gault, Joseph.,Donlan, Joseph A. C..,Liko, Idlir.,Hopper, Jonathan T. S..,Gupta, Kallol.,...&Robinson, Carol V..(2016).High-resolution mass spectrometry of small molecules bound to membrane proteins.NATURE METHODS,13(4),333-336. |
| MLA | Gault, Joseph,et al."High-resolution mass spectrometry of small molecules bound to membrane proteins".NATURE METHODS 13.4(2016):333-336. |
入库方式： OAI收割
来源：上海药物研究所
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