Destabilization of strigolactone receptor DWARF14 by binding of ligand and E3-ligase signaling effector DWARF3
文献类型：期刊论文
| 作者 | Zhao, Li-Hua5,10,11; Zhou, X. Edward6,7; Yi, Wei10; Wu, Zhongshan6,7,8,10; Liu, Yue10; Kang, Yanyong6,7; Hou, Li6,7,10 ; de Waal, Parker W.6,7; Li, Suling10; Jiang, Yi10
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| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2015-11 |
| 卷号 | 25期号:11页码:1219-1236 |
| 关键词 | Strigolactones D14 D3 GR24 receptor/co-receptor |
| ISSN号 | 1001-0602 |
| DOI | 10.1038/cr.2015.122 |
| 文献子类 | Article |
| 英文摘要 | Strigolactones (SLs) are endogenous hormones and exuded signaling molecules in plant responses to low levels of mineral nutrients. Key mediators of the SL signaling pathway in rice include the alpha/beta-fold hydrolase DWARF 14 (D14) and the F-box component DWARF 3 (D3) of the ubiquitin ligase SCFD3 that mediate ligand-dependent degradation of downstream signaling repressors. One perplexing feature is that D14 not only functions as the SL receptor but is also an active enzyme that slowly hydrolyzes diverse natural and synthetic SLs including GR24, preventing the crystallization of a binary complex of D14 with an intact SL as well as the ternary D14/SL/D3 complex. Here we overcome these barriers to derive a structural model of D14 bound to intact GR24 and identify the interface that is required for GR24-mediated D14-D3 interaction. The mode of GR24-mediated signaling, including ligand recognition, hydrolysis by D14, and ligand-mediated D14-D3 interaction, is conserved in structurally diverse SLs. More importantly, D14 is destabilized upon the binding of ligands and D3, thus revealing an unusual mechanism of SL recognition and signaling, in which the hormone, the receptor, and the downstream effectors are systematically destabilized during the signal transduction process. |
| WOS关键词 | MACROMOLECULAR STRUCTURES ; PLANT DEVELOPMENT ; ARABIDOPSIS ; KARRIKIN ; RICE ; DEGRADATION ; INHIBITION ; PERCEPTION ; SOFTWARE ; RECOGNITION |
| 资助项目 | Van Andel Research Institute, Amway (China)[00000000] ; National Natural Science Foundation of China[NSFC 31300245] ; National Natural Science Foundation of China[NSFC 91217311] ; Ministry of Science and Technology of China[2012ZX09301001] ; Ministry of Science and Technology of China[2012CB910403] ; Ministry of Science and Technology of China[2013CB910600] ; Ministry of Science and Technology of China[XDB08020303] ; Ministry of Science and Technology of China[2013ZX09507001] ; National Institute of Health (USA)[DK071662] ; National Institute of Health (USA)[GM102545] ; National Institute of Health (USA)[GM104212] ; Michigan Economic Development Corporation[00000000] ; Michigan Technology Tri-Corridor[085P1000817] ; Office of Science of the US Department of Energy[DE-AC02-06CH11357] |
| WOS研究方向 | Cell Biology |
| 语种 | 英语 |
| CSCD记录号 | CSCD:5558217 |
| WOS记录号 | WOS:000365049100008 |
| 出版者 | INST BIOCHEMISTRY & CELL BIOLOGY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276341]  |
| 专题 | 药物靶标结构与功能中心 |
| 通讯作者 | Melcher, Karsten |
| 作者单位 | 1.Univ Tasmania, Sch Biol Sci, Hobart, Tas 7001, Australia; 2.Chinese Acad Sci, State Key Lab Plant Genom, Beijing 100101, Peoples R China; 3.Chinese Acad Sci, Natl Ctr Plant Gene Res Beijing, Inst Genet & Dev Biol, Beijing 100101, Peoples R China; 4.Scripps Res Inst, Translat Res Inst, Dept Mol Therapeut, Jupiter, FL 33458 USA 5.E China Normal Univ, Inst Biomed Sci, Shanghai 200241, Peoples R China; 6.Van Andel Res Inst, Lab Struct Sci, Grand Rapids, MI USA; 7.Van Andel Res Inst, Lab Struct Biol & Biochem, Grand Rapids, MI USA; 8.Huazhong Univ Sci & Technol, Wuhan Natl Lab Optoelect, Britton Chance Ctr Biomed Photon, Wuhan 430074, Hubei, Peoples R China; 9.Univ Western Australia, Sch Chem & Biochem, Crawley, WA 6009, Australia; 10.Chinese Acad Sci, Ctr Struct & Funct Drug Targets, Key Lab Receptor Res, VARI SIMM Ctr,Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Zhao, Li-Hua,Zhou, X. Edward,Yi, Wei,et al. Destabilization of strigolactone receptor DWARF14 by binding of ligand and E3-ligase signaling effector DWARF3[J]. CELL RESEARCH,2015,25(11):1219-1236. |
| APA | Zhao, Li-Hua.,Zhou, X. Edward.,Yi, Wei.,Wu, Zhongshan.,Liu, Yue.,...&Xu, H. Eric.(2015).Destabilization of strigolactone receptor DWARF14 by binding of ligand and E3-ligase signaling effector DWARF3.CELL RESEARCH,25(11),1219-1236. |
| MLA | Zhao, Li-Hua,et al."Destabilization of strigolactone receptor DWARF14 by binding of ligand and E3-ligase signaling effector DWARF3".CELL RESEARCH 25.11(2015):1219-1236. |
入库方式： OAI收割
来源：上海药物研究所
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