Structural basis of JAZ repression of MYC transcription factors in jasmonate signalling
文献类型：期刊论文
| 作者 | Zhang, Feng2,3,4,5; Yao, Jian4,6; Ke, Jiyuan2,3; Zhang, Li4,7; Lam, Vinh Q.8; Xin, Xiu-Fang4; Zhou, X. Edward2,3; Chen, Jian2,3,9; Brunzelle, Joseph10; Griffin, Patrick R.8 |
| 刊名 | NATURE
 |
| 出版日期 | 2015-09-10 |
| 卷号 | 525期号:7568页码:269-+ |
| ISSN号 | 0028-0836 |
| DOI | 10.1038/nature14661 |
| 文献子类 | Article |
| 英文摘要 | The plant hormone jasmonate plays crucial roles in regulating plant responses to herbivorous insects and microbial pathogens and is an important regulator of plant growth and development(1-7). Keymediators of jasmonate signalling include MYC transcription factors, which are repressed by jasmonate ZIM-domain (JAZ) transcriptional repressors in the resting state. In the presence of active jasmonate, JAZ proteins function as jasmonate co-receptors by forming a hormone-dependent complex with COI1, the F-box subunit of an SCF-type ubiquitin E3 ligase(8-11). The hormone-dependent formation of the COI1-JAZ co-receptor complex leads to ubiquitination and proteasome-dependent degradation of JAZ repressors and release of MYC proteins from transcriptional repression(3,10,12). The mechanism by which JAZ proteins repress MYC transcription factors and how JAZ proteins switch between the repressor function in the absence of hormone and the co-receptor function in the presence of hormone remain enigmatic. Here we show that Arabidopsis MYC3 undergoes pronounced conformational changes when bound to the conserved Jas motif of the JAZ9 repressor. The Jas motif, previously shown to bind to hormone as a partly unwound helix, forms a complete alpha-helix that displaces the amino (N)-terminal helix ofMYC3 and becomes an integral part of theMYC N-terminal fold. In this position, the Jas helix competitively inhibits MYC3 interaction with the MED25 subunit of the transcriptional Mediator complex. Our structural and functional studies elucidate a dynamic molecular switchmechanism that governs the repression and activation of a major plant hormone pathway. |
| WOS关键词 | ACTIVATION DOMAINS ; GENE-EXPRESSION ; ARABIDOPSIS ; PROTEINS ; CORONATINE ; MEDIATOR ; RECEPTOR ; DEFENSE ; COMPLEX ; RESPONSES |
| 资助项目 | Gordon and Betty Moore Foundation[GBMF3037] ; China Scholarship Council[00000000] ; Van Andel Research Institute[00000000] ; National Institutes of Health[R01 GM102545] ; National Institutes of Health[R01AI060761] ; Department of Energy (the Chemical Sciences, Geosciences, and Biosciences Division, Office of Basic Energy Sciences, Office of Science)[DE-FG02-91ER20021] ; Michigan Economic Development Corporation[00000000] ; Michigan Technology Tri-Corridor[085P1000817] ; Office of Science of the US Department of Energy[DE-AC02-06CH11357] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000360927400043 |
| 出版者 | NATURE PUBLISHING GROUP |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276400]  |
| 专题 | 药物靶标结构与功能中心 |
| 通讯作者 | Xu, H. Eric |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Inst Biol Sci, Key Lab Receptor Res,VARI SIMM Ctr,Ctr Struct & F, Shanghai 200031, Peoples R China; 2.Van Andel Res Inst, Lab Struct Sci, Grand Rapids, MI 49503 USA; 3.Van Andel Res Inst, Lab Struct Biol & Biochem, Grand Rapids, MI 49503 USA; 4.Michigan State Univ, DOE Plant Res Lab, E Lansing, MI 48824 USA; 5.Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China; 6.Western Michigan Univ, Dept Biol Sci, Kalamazoo, MI 49008 USA; 7.Michigan State Univ, Dept Plant Biol, E Lansing, MI 48824 USA; 8.Scripps Florida, Scripps Res Inst, Translat Res Inst, Dept Mol Therapeut, Jupiter, FL 33458 USA; 9.Zhejiang Sci Tech Univ, Coll Life Sci, Hangzhou 310018, Zhejiang, Peoples R China; 10.Northwestern Univ, Synchrotron Res Ctr, Dept Mol Pharmacol & Biol Chem, Life Sci Collaborat Access Team, Argonne, IL 60439 USA; |
| 推荐引用方式 GB/T 7714 | Zhang, Feng,Yao, Jian,Ke, Jiyuan,et al. Structural basis of JAZ repression of MYC transcription factors in jasmonate signalling[J]. NATURE,2015,525(7568):269-+. |
| APA | Zhang, Feng.,Yao, Jian.,Ke, Jiyuan.,Zhang, Li.,Lam, Vinh Q..,...&He, Sheng Yang.(2015).Structural basis of JAZ repression of MYC transcription factors in jasmonate signalling.NATURE,525(7568),269-+. |
| MLA | Zhang, Feng,et al."Structural basis of JAZ repression of MYC transcription factors in jasmonate signalling".NATURE 525.7568(2015):269-+. |
入库方式： OAI收割
来源：上海药物研究所
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