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Structural basis for recognition of diverse transcriptional repressors by the TOPLESS family of corepressors

文献类型：期刊论文

作者Ke, Jiyuan1,2,6; Ma, Honglei1,2,6; Gu, Xin1,6; Thelen, Adam1,6; Brunzelle, Joseph S.5; Li, Jiayang3,4; Xu, H. Eric1,2,6; Melcher, Karsten1,2,6
刊名SCIENCE ADVANCES
出版日期2015-07
卷号1期号:6
ISSN号2375-2548
DOI10.1126/sciadv.1500107
文献子类Article
英文摘要TOPLESS (TPL) and TOPLESS-related (TPR) proteins comprise a conserved family of plant transcriptional corepressors that are related to Tup1, Groucho, and TLE (transducin-like enhancer of split) corepressors in yeast, insects, and mammals. In plants, TPL/TPR corepressors regulate development, stress responses, and hormone signaling through interaction with small ethylene response factor-associated amphiphilic repression (EAR) motifs found in diverse transcriptional repressors. How EAR motifs can interact with TPL/TPR proteins is unknown. We confirm the amino-terminal domain of the TPL family of corepressors, which we term TOPLESS domain (TPD), as the EAR motifbinding domain. To understand the structural basis of this interaction, we determined the crystal structures of the TPD of rice (Os) TPR2 in apo (apo protein) state and in complexes with the EAR motifs from Arabidopsis NINJA (novel interactor of JAZ), IAA1 (auxin-responsive protein 1), and IAA10, key transcriptional repressors involved in jasmonate and auxin signaling. The OsTPR2 TPD adopts a new fold of nine helices, followed by a zinc finger, which are arranged into a disc-like tetramer. The EAR motifs in the three different complexes adopt a similar extended conformation with the hydrophobic residues fitting into the same surface groove of each OsTPR2 monomer. Sequence alignments and structure-based mutagenesis indicate that this mode of corepressor binding is highly conserved in a large set of transcriptional repressors, thus providing a general mechanism for gene repression mediated by the TPL family of corepressors.
资助项目Van Andel Research Institute[00000000] ; Ministry of Science and Technology (People's Republic of China)[2012ZX09301001] ; Ministry of Science and Technology (People's Republic of China)[2012CB910403] ; Ministry of Science and Technology (People's Republic of China)[2013CB910600] ; Ministry of Science and Technology (People's Republic of China)[XDB08020303] ; Ministry of Science and Technology (People's Republic of China)[2013ZX09507001] ; NSF[91217311] ; NIH[DK071662] ; NIH[GM102545] ; NIH[GM104212] ; Michigan Economic Development Corporation and the Michigan Technology Tri-Corridor[085P1000817]
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:000216595400016
出版者AMER ASSOC ADVANCEMENT SCIENCE
源URL[http://119.78.100.183/handle/2S10ELR8/276487]  
专题药物靶标结构与功能中心
通讯作者Xu, H. Eric
作者单位1.Van Andel Res Inst, Lab Struct Sci, Grand Rapids, MI 49503 USA;
2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Key Lab Receptor Res,VARI SIMM Ctr,Ctr Struct & F, Shanghai 201203, Peoples R China;
3.Chinese Acad Sci, Inst Genet & Dev Biol, Natl Ctr Plant Gene Res Beijing, Beijing 100101, Peoples R China
4.Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Genom, Beijing 100101, Peoples R China;
5.Northwestern Univ, Synchrotron Res Ctr, Life Sci Collaborat Access Team, Dept Mol Pharmacol & Biol Chem, Argonne, IL 60439 USA;
6.Van Andel Res Inst, Lab Struct Biol & Biochem, Grand Rapids, MI 49503 USA;
推荐引用方式
GB/T 7714		 Ke, Jiyuan,Ma, Honglei,Gu, Xin,et al. Structural basis for recognition of diverse transcriptional repressors by the TOPLESS family of corepressors[J]. SCIENCE ADVANCES,2015,1(6).
APA Ke, Jiyuan.,Ma, Honglei.,Gu, Xin.,Thelen, Adam.,Brunzelle, Joseph S..,...&Melcher, Karsten.(2015).Structural basis for recognition of diverse transcriptional repressors by the TOPLESS family of corepressors.SCIENCE ADVANCES,1(6).
MLA Ke, Jiyuan,et al."Structural basis for recognition of diverse transcriptional repressors by the TOPLESS family of corepressors".SCIENCE ADVANCES 1.6(2015).

入库方式： OAI收割

来源：上海药物研究所

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