PGC-1 Coactivator Activity Is Required for Murine Erythropoiesis
文献类型：期刊论文
| 作者 | Cui, Shuaiying4; Tanabe, Osamu4,5; Lim, Kim-Chew4; Xu, H. Eric1,2 ; Zhou, X. Edward1; Lin, Jiandie D.4,6; Shi, Lihong4; Schmidt, Lindsay7; Campbell, Andrew9; Shimizu, Ritsuko3
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| 刊名 | MOLECULAR AND CELLULAR BIOLOGY
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| 出版日期 | 2014-06 |
| 卷号 | 34期号:11页码:1956-1965 |
| ISSN号 | 0270-7306 |
| DOI | 10.1128/MCB.00247-14 |
| 文献子类 | Article |
| 英文摘要 | Peroxisome proliferator-activated receptor gamma (PPAR gamma) coactivator 1 alpha (PGC-1 alpha) and PGC-1 beta have been shown to be intimately involved in the transcriptional regulation of cellular energy metabolism as well as other biological processes, but both coactivator proteins are expressed in many other tissues and organs in which their function is, in essence, unexplored. Here, we found that both PGC-1 proteins are abundantly expressed in maturing erythroid cells. PGC-1 alpha and PGC-1 beta compound null mutant (Pgc-1(c)) animals express less beta-like globin mRNAs throughout development; consequently, neonatal Pgc-1(c) mice exhibit growth retardation and profound anemia. Flow cytometry shows that the number of mature erythrocytes is markedly reduced in neonatal Pgc-1(c) pups, indicating that erythropoiesis is severely compromised. Furthermore, hematoxylin and eosin staining revealed necrotic cell death and cell loss in Pgc-1(c) livers and spleen. Chromatin immunoprecipitation studies revealed that both PGC-1 alpha and -1 beta, as well as two nuclear receptors, TR2 and TR4, coordinately bind to the various globin gene promoters. In addition, PGC-1 alpha and -1 beta can interact with TR4 to potentiate transcriptional activation. These data provide new insights into our understanding of globin gene regulation and raise the interesting possibility that the PGC-1 coactivators can interact with TR4 to elicit differential stage-specific effects on globin gene transcription. |
| WOS关键词 | FETAL-HEMOGLOBIN EXPRESSION ; ORPHAN NUCLEAR RECEPTORS ; SICKLE-CELL-DISEASE ; CNTFR-ALPHA GENE ; GLOBIN GENE ; TRANSCRIPTIONAL COACTIVATOR ; ENERGY-METABOLISM ; MITOCHONDRIAL BIOGENESIS ; HEPATIC GLUCONEOGENESIS ; HEREDITARY PERSISTENCE |
| 资助项目 | NIH[DK86956] ; NIH[HL24415] ; NIH[DK071662] ; NIH[DK066202] ; NIH[HL089301] ; NIH[DK077086] ; Jay and Betty Van Andel Foundation[00000000] ; Amway (China) Limited[00000000] ; American Heart Association for a Scientist Development Grant[00000000] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Cell Biology |
| 语种 | 英语 |
| 出版者 | AMER SOC MICROBIOLOGY |
| WOS记录号 | WOS:000335967100005 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277059]  |
| 专题 | 药物靶标结构与功能中心 |
| 通讯作者 | Engel, James Douglas |
| 作者单位 | 1.Van Andel Res Inst, Lab Struct Sci, Grand Rapids, MI USA; 2.Chinese Acad Sci, Shanghai Inst Mat Med, Key Lab Receptor Res, VARI SIMM Ctr,Ctr Struct & Funct Drug Targets, Shanghai 200031, Peoples R China; 3.Tohoku Univ, Grad Sch Med, Dept Mol Hematol, Sendai, Miyagi 980, Japan; 4.Univ Michigan, Sch Med, Dept Cell & Dev Biol, Ann Arbor, MI 48109 USA; 5.Tohoku Univ, Tohoku Med Megabank, Dept Integrat Genom, Sendai, Miyagi 980, Japan 6.Univ Michigan, Sch Med, Inst Life Sci, Ann Arbor, MI USA; 7.Univ Michigan, Sch Med, Dept Pathol, Ann Arbor, MI USA; 8.Tohoku Univ, Grad Sch Med, Dept Med Biochem, Sendai, Miyagi 980, Japan; 9.Univ Michigan, Sch Med, Dept Pediat & Infect Dis, Ann Arbor, MI USA; |
| 推荐引用方式 GB/T 7714 | Cui, Shuaiying,Tanabe, Osamu,Lim, Kim-Chew,et al. PGC-1 Coactivator Activity Is Required for Murine Erythropoiesis[J]. MOLECULAR AND CELLULAR BIOLOGY,2014,34(11):1956-1965. |
| APA | Cui, Shuaiying.,Tanabe, Osamu.,Lim, Kim-Chew.,Xu, H. Eric.,Zhou, X. Edward.,...&Engel, James Douglas.(2014).PGC-1 Coactivator Activity Is Required for Murine Erythropoiesis.MOLECULAR AND CELLULAR BIOLOGY,34(11),1956-1965. |
| MLA | Cui, Shuaiying,et al."PGC-1 Coactivator Activity Is Required for Murine Erythropoiesis".MOLECULAR AND CELLULAR BIOLOGY 34.11(2014):1956-1965. |
入库方式： OAI收割
来源：上海药物研究所
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