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Structural basis for RNA recognition by a dimeric PPR-protein complex

文献类型：期刊论文

作者Ke, Jiyuan6; Chen, Run-Ze5; Ban, Ting1,5; Zhou, X. Edward6; Gu, Xin6; Tan, M. H. Eileen4,6; Chen, Chen4,6; Kang, Yanyong6; Brunzelle, Joseph S.3; Zhu, Jian-Kang1,2
刊名NATURE STRUCTURAL & MOLECULAR BIOLOGY
出版日期2013-12
卷号20期号:12页码:1377-1382
ISSN号1545-9993
DOI10.1038/nsmb.2710
文献子类Article
英文摘要Thylakoid assembly 8 (THA8) is a pentatricopeptide repeat (PPR) RNA-binding protein required for the splicing of the transcript of ycf3, a gene involved in chloroplast thylakoid-membrane biogenesis. Here we report the identification of multiple THA8-binding sites in the ycf3 intron and present crystal structures of Brachypodium distachyon THA8 either free of RNA or bound to two of the identified RNA sites. The apostructure reveals a THA8 monomer with five tandem PPR repeats arranged in a planar fold. The complexes of THA8 bound to the two short RNA fragments surprisingly reveal asymmetric THA8 dimers with the bound RNAs at the dimeric interface. RNA binding induces THA8 dimerization, with a conserved G nucleotide of the bound RNAs making extensive contacts with both monomers. Together, these results establish a new model of RNA recognition by RNA-induced formation of an asymmetric dimer of a PPR protein.
WOS关键词PENTATRICOPEPTIDE REPEAT PROTEINS ; CHLOROPLASTS ; BINDING ; MECHANISM ; TERMINI ; DNA
资助项目Michigan Economic Development Corporation[00000000] ; Michigan Technology Tri-Corridor[085P1000817] ; Office of Science of the US Department of Energy[DE-AC02-06CH11357] ; Jay and Betty Van Andel Foundation[00000000] ; Ministry of Science and Technology (China)[2012ZX09301001-005] ; Ministry of Science and Technology (China)[2012CB910403] ; Amway (China)[00000000] ; US National Institutes of Health[R01 DK071662] ; Chinese Academy of Sciences[00000000]
WOS研究方向Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
语种英语
出版者NATURE PUBLISHING GROUP
WOS记录号WOS:000328007600009
源URL[http://119.78.100.183/handle/2S10ELR8/277357]  
专题药物靶标结构与功能中心
通讯作者Xu, H. Eric
作者单位1.Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, Shanghai Inst Plant Physiol & Ecol, Shanghai Inst Biol Sci, Shanghai, Peoples R China;
2.Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47907 USA
3.Northwestern Univ, Synchrotron Res Ctr, Life Sci Collaborat Access Team, Argonne, IL USA;
4.Natl Univ Singapore, Yong Loo Lin Sch Med, Natl Univ Hosp, Dept Obstet & Gynecol, Singapore 117595, Singapore;
5.Chinese Acad Sci, Shanghai Inst Mat Med, Key Lab Receptor Res,Ctr Struct & Funct Drug Targ, Shanghai Inst Mat Med VARI SIMM Ctr,Van Andel Res, Shanghai 200031, Peoples R China;
6.Van Andel Res Inst, Ctr Struct Biol & Drug Discovery, Lab Struct Sci, Grand Rapids, MI USA;
推荐引用方式
GB/T 7714		 Ke, Jiyuan,Chen, Run-Ze,Ban, Ting,et al. Structural basis for RNA recognition by a dimeric PPR-protein complex[J]. NATURE STRUCTURAL & MOLECULAR BIOLOGY,2013,20(12):1377-1382.
APA Ke, Jiyuan.,Chen, Run-Ze.,Ban, Ting.,Zhou, X. Edward.,Gu, Xin.,...&Xu, H. Eric.(2013).Structural basis for RNA recognition by a dimeric PPR-protein complex.NATURE STRUCTURAL & MOLECULAR BIOLOGY,20(12),1377-1382.
MLA Ke, Jiyuan,et al."Structural basis for RNA recognition by a dimeric PPR-protein complex".NATURE STRUCTURAL & MOLECULAR BIOLOGY 20.12(2013):1377-1382.

入库方式： OAI收割

来源：上海药物研究所

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