Ice breaking in GPCR structural biology
文献类型：期刊论文
| 作者 | Zhao, Qiang ; Wu, Bei-li
|
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2012-03 |
| 卷号 | 33期号:3页码:324-334 |
| 关键词 | G-protein-coupled receptors (GPCRs) expression study mutagenesis surfactant crystallization bicelle lipidic cubic phase |
| ISSN号 | 1671-4083 |
| DOI | 10.1038/aps.2011.187 |
| 文献子类 | Review |
| 英文摘要 | G-protein-coupled receptors (GPCRs) are one of the most challenging targets in structural biology. To successfully solve a high-resolution GPCR structure, several experimental obstacles must be overcome, including expression, extraction, purification, and crystallization. As a result, there are only a handful of unique structures reported from this protein superfamily, which consists of over 800 members. In the past few years, however, there has been an increase in the amount of solved GPCR structures, and a few high-impact structures have been determined: the peptide receptor CXCR4, the agonist bound receptors, and the GPCR-G protein complex. The dramatic progress in GPCR structural studies is not due to the development of any single technique, but a combination of new techniques, new tools and new concepts. Here, we summarize the progress made for GPCR expression, purification, and crystallization, and we highlight the technical advances that will facilitate the future determination of GPCR structures. |
| WOS关键词 | PROTEIN-COUPLED RECEPTOR ; CRYSTALLIZING MEMBRANE-PROTEINS ; MU-OPIOID RECEPTOR ; YEAST PICHIA-PASTORIS ; ADENOSINE A(2A) RECEPTOR ; LIPIDIC CUBIC PHASES ; SACCHAROMYCES-CEREVISIAE ; HETEROLOGOUS EXPRESSION ; PHARMACOLOGICAL CHARACTERIZATION ; NEUROTENSIN RECEPTOR |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| CSCD记录号 | CSCD:4455248 |
| WOS记录号 | WOS:000301187300005 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278167]  |
| 专题 | 药物靶标结构与功能中心 |
| 通讯作者 | Zhao, Qiang |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, Joint Labs GPCR Res, Ctr Struct & Funct Drug Targets, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Qiang,Wu, Bei-li. Ice breaking in GPCR structural biology[J]. ACTA PHARMACOLOGICA SINICA,2012,33(3):324-334. |
| APA | Zhao, Qiang,&Wu, Bei-li.(2012).Ice breaking in GPCR structural biology.ACTA PHARMACOLOGICA SINICA,33(3),324-334. |
| MLA | Zhao, Qiang,et al."Ice breaking in GPCR structural biology".ACTA PHARMACOLOGICA SINICA 33.3(2012):324-334. |
入库方式： OAI收割
来源：上海药物研究所
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