中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 上海药物研究所
  3. 中国科学院上海药物研究所
  4. 药物靶标结构与功能中心
Cryo-EM structure of human rhodopsin bound to an inhibitory G protein

文献类型：期刊论文

作者Kang, Yanyong7; Kuybeda, Oleg6; de Waal, Parker W.7; Mukherjee, Somnath5; Van Eps, Ned4; Dutka, Przemyslaw3,5; Zhou, X. Edward7; Bartesaghi, Alberto6; Erramilli, Satchal5; Morizumi, Takefumi4
刊名NATURE
出版日期2018-06-28
卷号558期号:7711页码:553-+
ISSN号0028-0836
DOI10.1038/s41586-018-0215-y
文献子类Article
英文摘要G-protein-coupled receptors comprise the largest family of mammalian transmembrane receptors. They mediate numerous cellular pathways by coupling with downstream signalling transducers, including the hetrotrimeric G proteins G(s) (stimulatory) and G(i) (inhibitory) and several arrestin proteins. The structural mechanisms that define how G-protein-coupled receptors selectively couple to a specific type of G protein or arrestin remain unknown. Here, using cryo-electron microscopy, we show that the major interactions between activated rhodopsin and G(i) are mediated by the C-terminal helix of the G(i) alpha-subunit, which is wedged into the cytoplasmic cavity of the transmembrane helix bundle and directly contacts the amino terminus of helix 8 of rhodopsin. Structural comparisons of inactive, G(i)-bound and arrestin-bound forms of rhodopsin with inactive and G(s)-bound forms of the beta(2)-adrenergic receptor provide a foundation to understand the unique structural signatures that are associated with the recognition of G(s), G(i) and arrestin by activated G-protein-coupled receptors.
WOS关键词CATALYZED NUCLEOTIDE EXCHANGE ; LIGHT-ACTIVATED RHODOPSIN ; HETEROTRIMERIC G-PROTEINS ; COUPLED RECEPTORS ; CRYSTAL-STRUCTURE ; ALPHA-SUBUNITS ; BINDING-SITES ; TRANSDUCIN ; COMPLEX ; INSIGHTS
资助项目National Institutes of Health[DK071662] ; American Asthma Foundation[00000000] ; Jay and Betty Van Andel Foundation[00000000] ; Ministry of Science and Technology (China)[2012ZX09301001] ; Ministry of Science and Technology (China)[2012CB910403] ; Ministry of Science and Technology (China)[2013CB910600] ; Ministry of Science and Technology (China)[XDB08020303] ; Ministry of Science and Technology (China)[2013ZX09507001] ; Ministry of Science and Technology (China)[GM117372] ; Ministry of Science and Technology (China)[GM0875119] ; Pfizer[00000000] ; National Natural Science Foundation[31770796] ; Canada Excellence Research Chairs program[00000000] ; Canadian Institute for Advanced Research[00000000] ; Anne and Max Tanenbaum Chair in Neuroscience[00000000] ; Center for Cancer Research, National Cancer Institute, NIH, Bethesda, MD[00000000] ; Frederick National Laboratory for Cancer Research, National Institutes of Health[HHSN261200800001E]
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:000436594300053
出版者NATURE PUBLISHING GROUP
源URL[http://119.78.100.183/handle/2S10ELR8/279695]  
专题药物靶标结构与功能中心
通讯作者Kossiakoff, Anthony A.; Subramaniam, Sriram; Xu, H. Eric
作者单位1.Univ Chicago, Inst Biophys Dynam, Chicago, IL 60637 USA;
2.Univ Chinese Acad Sci, Beijing, Peoples R China;
3.Jagiellonian Univ, Fac Biochem Biophys & Biotechnol, Krakow, Poland;
4.Univ Toronto, Dept Biochem, Toronto, ON, Canada;
5.Univ Chicago, Dept Biochem & Mol Biol, 920 E 58Th St, Chicago, IL 60637 USA;
6.Frederick Natl Lab Canc Res, Canc Res Technol Program, Frederick, MD 21702 USA;
7.Van Andel Res Inst, Innovat & Integrat Program, Ctr Canc & Cell Biol, Grand Rapids, MI 49503 USA;
8.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Struct & Funct Drug Targets, VARI SIMM Ctr,Key Lab Receptor Res, Shanghai, Peoples R China;
9.Van Andel Res Inst, David Van Andel Adv Cryoelect Microscopy Suite, Grand Rapids, MI USA;
10.Univ Toronto, Dept Mol Genet, Toronto, ON, Canada;
推荐引用方式
GB/T 7714		 Kang, Yanyong,Kuybeda, Oleg,de Waal, Parker W.,et al. Cryo-EM structure of human rhodopsin bound to an inhibitory G protein[J]. NATURE,2018,558(7711):553-+.
APA Kang, Yanyong.,Kuybeda, Oleg.,de Waal, Parker W..,Mukherjee, Somnath.,Van Eps, Ned.,...&Xu, H. Eric.(2018).Cryo-EM structure of human rhodopsin bound to an inhibitory G protein.NATURE,558(7711),553-+.
MLA Kang, Yanyong,et al."Cryo-EM structure of human rhodopsin bound to an inhibitory G protein".NATURE 558.7711(2018):553-+.

入库方式： OAI收割

来源：上海药物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。