The effect of processing medium on the H-2/H-1 of carbon-bound hydrogen in alpha-cellulose extracted from higher plants
文献类型：期刊论文
| 作者 | Zhu, Zhenyu1,4,6; Yin, Xijie3; Lu, Fengyan2; Wang, Bo4,6; Ma, Ran4,6; Zhao, Yu4,6; Wang, Ying4,6; Ma, Yi4,6; Su, Jing3; Yan, Qiulin4,6 |
| 刊名 | RAPID COMMUNICATIONS IN MASS SPECTROMETRY
 |
| 出版日期 | 2020-05-30 |
| 卷号 | 34期号:10页码:12 |
| ISSN号 | 0951-4198 |
| DOI | 10.1002/rcm.8641 |
| 通讯作者 | Zhou, Youping(youping.zhou@sust.edu.cn) |
| 英文摘要 | Rationale Although the H-2/H-1 ratio of the carbon-bound hydrogens (C-Hs) in alpha-cellulose extracted from higher plants has long been used successfully for climate, environmental and metabolic studies, the assumption that bleaching with acidified NaClO2 to remove lignin before pure alpha-cellulose can be obtained does not alter the H-2/H-1 ratio of alpha-cellulose C-Hs has nonetheless not been tested. Methods For reliable application of the H-2/H-1 ratio of alpha-cellulose C-H, we processed plant materials representing different phytochemistries and photosynthetic carbon assimilation modes in isotopically contrasting bleaching media (with an isotopic difference of 273 mUr). All the isotope ratios were measured by elemental analyzer/isotope ratio mass spectrometry (EA/IRMS). Results Our results show that H from the bleaching medium does appear in the final pure alpha-cellulose product, although the isotopic alteration to the C-H in alpha-cellulose due to the incorporation of processing H from the medium is small if isotopically "natural" water is used to prepare the processing medium. However, under prolonged bleaching such an isotope effect can be significant, implying that standardizing the bleaching process is necessary for reliable H-2/H-1 measurement. Conclusions The currently adopted method for removing lignin for alpha-cellulose extraction from higher plant materials with acidified NaClO2 bleaching is considered acceptable in terms of preserving the isotopic fidelity if isotopically "natural" water is used to prepare the bleaching solution. |
| WOS关键词 | CONTAINING CHEMICAL LINKAGES ; DELTA-D VALUES ; D/H RATIOS ; NONEXCHANGEABLE HYDROGEN ; TREE-RINGS ; OXYGEN ; FRACTIONATION ; LIGNIN ; EXCHANGE ; WATER |
| 资助项目 | Chinese NSF[41773032] ; Chinese NSF[41973072] ; Shaanxi Natural Science Basic Research Plan[2019JQ-232] ; Shaanxi Natural Science Basic Research Plan[037] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:000529873700017 |
| 资助机构 | Chinese NSF ; Shaanxi Natural Science Basic Research Plan |
| 源URL | [http://ir.ieecas.cn/handle/361006/14754]  |
| 专题 | 地球环境研究所_现代环境研究室 |
| 通讯作者 | Zhou, Youping |
| 作者单位 | 1.Southern Marine Sci & Engn Guangdong Lab, Zhuhai 519000, Peoples R China 2.Chinese Acad Sci, Inst Earth Environm, Xian 710061, Peoples R China 3.MNR Third Inst Oceanol, Lab Marine & Coastal Geol, Xiamen 361005, Peoples R China 4.Shaanxi Univ Sci & Technol, Sch Chem & Chem Engn, Shaanxi Key Lab Chem Addit Ind, Weiyang Univ Pk, Xian 710021, Peoples R China 5.Australian Natl Univ, Res Sch Biol, GPO Box 4, Canberra, ACT 2601, Australia 6.Shaanxi Univ Sci & Technol, Sch Chem & Chem Engn, Isotop Chem Biol, Weiyang Univ Pk, Xian 710021, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Zhenyu,Yin, Xijie,Lu, Fengyan,et al. The effect of processing medium on the H-2/H-1 of carbon-bound hydrogen in alpha-cellulose extracted from higher plants[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2020,34(10):12. |
| APA | Zhu, Zhenyu.,Yin, Xijie.,Lu, Fengyan.,Wang, Bo.,Ma, Ran.,...&Zhou, Youping.(2020).The effect of processing medium on the H-2/H-1 of carbon-bound hydrogen in alpha-cellulose extracted from higher plants.RAPID COMMUNICATIONS IN MASS SPECTROMETRY,34(10),12. |
| MLA | Zhu, Zhenyu,et al."The effect of processing medium on the H-2/H-1 of carbon-bound hydrogen in alpha-cellulose extracted from higher plants".RAPID COMMUNICATIONS IN MASS SPECTROMETRY 34.10(2020):12. |
入库方式： OAI收割
来源：地球环境研究所
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