A Chemical Strategy for Amphiphile Replacement in Membrane Protein Research
文献类型：期刊论文
| 作者 | Xue, Dongxian2,3,4,6; Wang, Jingjing2,4,5,6; Song, Xiyong4,7; Wang, Wei1; Hu, Tao2,3,4,6; Ye, Lintao2; Liu, Yang2; Zhou, Qingtong2; Zhou, Fang2,3,4,6; Jiang, Zhong-Xing1 |
| 刊名 | LANGMUIR
 |
| 出版日期 | 2019-03-26 |
| 卷号 | 35期号:12页码:4319-4327 |
| ISSN号 | 0743-7463 |
| DOI | 10.1021/acs.langmuir.8b04072 |
| 通讯作者 | Liu, Zhi-Jie(liuzhj@shanghaitech.edu.cn) ; Tao, Houchao(taohch@shanghaitech.edu.cn) |
| 英文摘要 | Membrane mimics are indispensable tools in the structural and functional understanding of membrane proteins (MPs). Given stringent requirements of integral MP manipulations, amphiphile replacement is often required in sample preparation for various biophysical purposes. Current protocols generally rely on physical methodologies and rarely reach complete replacement. In comparison, we report herein a chemical alternative that facilitates the exhaustive exchange of membrane-mimicking systems for MP reconstitution. This method, named sacrifice-replacement strategy, was enabled by a class of chemically cleavable detergents (CCDs), derived from the disulfide incorporation in the traditional detergent n-dodecyl-beta-D-maltopyranoside. The representative CCD behaved well in both solubilizing the diverse alpha-helical human G protein coupled receptors and refolding of the beta-barrel bacterial outer membrane protein X, and more importantly, it could also be readily degraded under mild conditions. By this means, the A(2A) adenosine receptor was successfully reconstituted into a series of commercial detergents for stabilization screening and nanodiscs for electron microscopy analysis. Featured by the simplicity and compatibility, this CCD-mediated strategy would later find more applications when being integrated in other biophysics studies. |
| WOS关键词 | PHOSPHOLIPID-BILAYER NANODISCS ; DETERGENTS ; QUANTIFICATION ; MECHANISMS ; DIGESTION ; COMPLEX ; OMPX |
| 资助项目 | Shanghai Municipal Government ; ShanghaiTech University ; NSFC[21672147] ; National Key R&D Program of China[2018YFA0507000] |
| WOS研究方向 | Chemistry ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000462944900014 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/290007]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Liu, Zhi-Jie; Tao, Houchao |
| 作者单位 | 1.Wuhan Univ, Sch Pharmaceut Sci, Hubei Prov Engn & Technol Res Ctr Fluorinated Pha, Wuhan 430071, Hubei, Peoples R China 2.ShanghaiTech Univ, iHuman Inst, Ren Bldg,393 Middle Huaxia Rd, Shanghai 201210, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China 4.Univ Chinese Acad Sci, 19A,Yuquan Rd, Beijing 100049, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China 6.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China 7.Chinese Acad Sci, Inst Biophys, Natl Lab Biomacromol, Beijing 100101, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xue, Dongxian,Wang, Jingjing,Song, Xiyong,et al. A Chemical Strategy for Amphiphile Replacement in Membrane Protein Research[J]. LANGMUIR,2019,35(12):4319-4327. |
| APA | Xue, Dongxian.,Wang, Jingjing.,Song, Xiyong.,Wang, Wei.,Hu, Tao.,...&Tao, Houchao.(2019).A Chemical Strategy for Amphiphile Replacement in Membrane Protein Research.LANGMUIR,35(12),4319-4327. |
| MLA | Xue, Dongxian,et al."A Chemical Strategy for Amphiphile Replacement in Membrane Protein Research".LANGMUIR 35.12(2019):4319-4327. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。