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Extraction, Characterization, Antitumor and Immunological Activities of Hemicellulose Polysaccharide from Astragalus radix Herb Residue

文献类型：期刊论文

作者Li, Ke2,3; Li, Shuying2,3,4; Wang, Di2,3,4; Li, Xiaoxia5; Wu, Xingkang2,3; Liu, Xiaojie2,3; Du, Guanhua6; Li, Xianrong1,7; Qin, Xuemei2,3; Du, Yuguang8
刊名MOLECULES
出版日期2019-10-01
卷号24期号:20页码:21
关键词Astragalus radix herb residue hemicellulose polysaccharide characterization antitumor immunomodulatory
DOI10.3390/molecules24203644
英文摘要Astragalus radix (radix) have been frequently used for clinical application in China, and the herb residues of radix turn out to be a waste of resources. To escape from this, the medicine value of radix herb residues is mined in this article. We isolated hemicellulose polysaccharide AX-I-3b from radix herb residues by fractional extraction. Monosaccharide-composition analysis revealed that AX-I-3b consisted of arabinose, xylose, and glucose with a molar ratio of 10.4:79.3:1.1. Methylation, NMR and FT-IR analyses showed that AX-I-3b monosaccharide residue was linked as follows: -> 2,3,4)-beta -d-Xylp-(1 ->, -> 4)-beta -d-Arap-(1 ->, -> 4)-beta -d-Glcp-(1 ->. Then, we found that AX-I-3b exhibited antitumor activity against lung cancer in vitro and vivo through MTT assay and xenograft tumor model. Mechanistically, AX-I-3b induced apoptosis in lung cancer cells and xenograft tumors, which is evidenced by the up-regulation of p53, Bax and cleaved caspase-3, and the down-regulation of Bcl-2. Moreover, AX-I-3b synergistically improved the therapeutic ability of cisplatin in xenograft tumors model. Furthermore, AX-I-3b treatment effectively improved the immune organ index, the percentage of spleen lymphocyte subsets and serum cytokine levels in lung cancer mice, supporting that AX-I-3b showed immunomodulatory activity. In conclusion, our results identified AX-I-3b as an antitumor and immunomodulatory agent, providing a new insight into the reutilization of radix herb residue.
WOS关键词STRUCTURAL-CHARACTERIZATION ; BCL-2 FAMILY ; IMMUNOMODULATORY ACTIVITY ; ANTIOXIDANT ACTIVITY ; IN-VITRO ; T-CELLS ; P53 ; APOPTOSIS ; MEMBERS ; ALKALI
资助项目National Natural Science Foundation of China[81872962] ; China Postdoctoral Science Foundation[2019M650851] ; Science and Technology Research Project of Shanxi Province[2014ZD0401] ; Key Projects of Key Research and Development Plan in Shanxi[201603D311101] ; Shanxi Province Technology Innovation Project of Excellent Talent[201605D211030] ; Shanxi Province Technology Innovation Project of Excellent Talent[201705D211020]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
WOS记录号WOS:000496249500013
出版者MDPI
资助机构National Natural Science Foundation of China ; China Postdoctoral Science Foundation ; Science and Technology Research Project of Shanxi Province ; Key Projects of Key Research and Development Plan in Shanxi ; Shanxi Province Technology Innovation Project of Excellent Talent
源URL[http://ir.ipe.ac.cn/handle/122111/39116]  
专题中国科学院过程工程研究所
通讯作者Qin, Xuemei; Du, Yuguang
作者单位1.Shanxi Acad Tradit Chinese Med, Taiyuan 030000, Shanxi, Peoples R China
2.Shanxi Univ, Modern Res Ctr Tradit Chinese Med, Taiyuan 030006, Shanxi, Peoples R China
3.Shanxi Univ, Engn Minist, Key Lab Chem Biol & Mol, Taiyuan 030006, Shanxi, Peoples R China
4.Shanxi Univ, Coll Chem & Chem Engn, Taiyuan 030006, Shanxi, Peoples R China
5.Shanxi Fruit Ind Work Stn, Taiyuan 030001, Shanxi, Peoples R China
6.Chinese Acad Med Sci, Inst Mat Med, Beijing 100050, Peoples R China
7.Shanxi Jianshuo Food & Drug Res Inst Co Ltd, Taiyuan 030000, Shanxi, Peoples R China
8.Chinese Acad Sci, Inst Proc Engn, Beijing 100190, Peoples R China
推荐引用方式
GB/T 7714		 Li, Ke,Li, Shuying,Wang, Di,et al. Extraction, Characterization, Antitumor and Immunological Activities of Hemicellulose Polysaccharide from Astragalus radix Herb Residue[J]. MOLECULES,2019,24(20):21.
APA Li, Ke.,Li, Shuying.,Wang, Di.,Li, Xiaoxia.,Wu, Xingkang.,...&Du, Yuguang.(2019).Extraction, Characterization, Antitumor and Immunological Activities of Hemicellulose Polysaccharide from Astragalus radix Herb Residue.MOLECULES,24(20),21.
MLA Li, Ke,et al."Extraction, Characterization, Antitumor and Immunological Activities of Hemicellulose Polysaccharide from Astragalus radix Herb Residue".MOLECULES 24.20(2019):21.

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来源：过程工程研究所

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