中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Chemical profiling of spermidines in goji berry by strong cation exchange solid-phase extraction (SCX-SPE) combined with ultrahigh-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS/MS)

文献类型:期刊论文

作者Ahad, H (Ahad, Hasanjan)[ 1,2,3 ]; Jin, HL (Jin, Hongli)[ 2 ]; Liu, YF (Liu, Yanfang)[ 2 ]; Wang, JX (Wang, Jixia)[ 2 ]; Sun, GY (Sun, Guangying)[ 1 ]; Liang, XM (Liang, Xinmiao)[ 2 ]; Aisa, HA (Aisa, Haji Akber)[ 1 ]
刊名JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
出版日期2020
卷号1137期号:1页码:1-10
ISSN号1570-0232
关键词Spermidines Fragmentation rules Goji berry Selective enrichment UPLC-DAD QTOF-MS MS
DOI10.1016/j.jchromb.2019.121923
英文摘要

Lycium barbarum fruit (Goji berry) have been used as a traditional Chinese medicine (TCM) with its outstanding biological and pharmacological activities. Spermidine alkaloids are a major class of bioactive constituents in goji berry, nevertheless, detailed information related to its identification remains scarce. In this study, chemical profiling of spermidines in goji berry was carried out by ultrahigh-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS). Four structure types of standards were used to study the comprehensive fragmentation rules of spermidines. Different types of spermidines were identified by distinctive MS/MS fragment ions. Noticeably, it was first proposed that the co-existence of fragment ions at m/z 220 and 222 was the key characteristic for distinguishing spermidine isomers. According to the structural feature of spermidines, a quick, convenient, highly selective strong cation exchange solid-phase extraction (SCX-SPE) combined with RP-LC procedure was developed for selective enrichment and the MS detection compatibility. A total of 41 out of 58 spermidines were tentatively characterized using the established method, of which 26 were reported for the first time from goji berry. This study provides guidelines and references for the identification of spermidines in natural products.

源URL[http://ir.xjipc.cas.cn/handle/365002/7237]  
专题新疆理化技术研究所_省部共建新疆特有药用资源利用重点实验室
新疆理化技术研究所_资源化学研究室
通讯作者Aisa, HA (Aisa, Haji Akber)[ 1 ]
作者单位1.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Plant Resources & Chem Arid Zone, Urumqi 830011, Xinjiang, Peoples R China
推荐引用方式
GB/T 7714
Ahad, H ,Jin, HL ,Liu, YF ,et al. Chemical profiling of spermidines in goji berry by strong cation exchange solid-phase extraction (SCX-SPE) combined with ultrahigh-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS/MS)[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2020,1137(1):1-10.
APA Ahad, H .,Jin, HL .,Liu, YF .,Wang, JX .,Sun, GY .,...&Aisa, HA .(2020).Chemical profiling of spermidines in goji berry by strong cation exchange solid-phase extraction (SCX-SPE) combined with ultrahigh-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS/MS).JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1137(1),1-10.
MLA Ahad, H ,et al."Chemical profiling of spermidines in goji berry by strong cation exchange solid-phase extraction (SCX-SPE) combined with ultrahigh-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS/MS)".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1137.1(2020):1-10.

入库方式: OAI收割

来源:新疆理化技术研究所

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