Isolation and Identification of Three Novel Antioxidant Peptides from the Bactrian Camel Milk Hydrolysates
文献类型：期刊论文
| 作者 | Wali, A (Wali, Ahmidin)[ 1,3 ]; Gao, YH (Gao Yanhua)[ 1 ]; Ishimov, U (Ishimov, Uchkun)[ 2 ]; Yili, A (Yili, Abulimiti)[ 1 ] ; Aisa, HA (Aisa, Haji Akber)[ 1 ]; Salikhov, S (Salikhov, Shavkat)[ 2 ]
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| 刊名 | INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS
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| 出版日期 | 2020 |
| 卷号 | 26期号:2页码:641-650 |
| 关键词 | Bactrian camel milk Enzymatic hydrolysis Antioxidant peptides Fractionation |
| ISSN号 | 1573-3149 |
| DOI | 10.1007/s10989-019-09871-x |
| 英文摘要 | The aim of this study was isolation and purification of antioxidant peptides from Bactrian camel milk (BCM) hydrolysate. Trypsin, pepsin, alcalase, and papain were used for hydrolysis of BCM. The peptides fraction from trypsin digest exhibited a strong antioxidant activity among them, and it was separated by ultrafiltration (3000 Da membrane). The resulting peptides (< 3000 Da) were further separated with using gel filtration chromatography (HW-55F) and reverse-phase high-performance liquid chromatography (RP-HPLC). Three novel antioxidant peptides were purified and sequenced by MALDI TOF-MS/MS as RLDGQGRPRVWLGR (TFI-b1), TPDNIDIWLGGIAEPQVKR (TFI-b2) and VAYSDDGENWTEYRDQGAVEGK (TFI-b3) with molecular weights 1665.94, 2122.13, and 2489.09 Da, respectively. The IC50 values of these peptides on DPPH were 1.9, 1.2 and 0.6 mg/mL and on ABTS are 2.4, 1.8 and 0.9 mg/mL, respectively. The peptides identified using similarity search tool of SIB ExPASy Bioinformatics Resources Portal (). |
| WOS记录号 | WOS:000529544100002 |
| 源URL | [http://ir.xjipc.cas.cn/handle/365002/7335]  |
| 专题 | 新疆理化技术研究所_省部共建新疆特有药用资源利用重点实验室 新疆理化技术研究所_资源化学研究室 |
| 通讯作者 | Yili, A (Yili, Abulimiti)[ 1 ] |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100039, Peoples R China 2.Uzbek Acad Sci, AS Sadykov Inst Bioorgan Chem, Tashkent 100125, Uzbekistan 3.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Plant Resources & Chem Arid Reg, Beijing South Rd 40-1, Urumqi 830011, Xinjiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wali, A ,Gao, YH ,Ishimov, U ,et al. Isolation and Identification of Three Novel Antioxidant Peptides from the Bactrian Camel Milk Hydrolysates[J]. INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS,2020,26(2):641-650. |
| APA | Wali, A ,Gao, YH ,Ishimov, U ,Yili, A ,Aisa, HA ,&Salikhov, S .(2020).Isolation and Identification of Three Novel Antioxidant Peptides from the Bactrian Camel Milk Hydrolysates.INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS,26(2),641-650. |
| MLA | Wali, A ,et al."Isolation and Identification of Three Novel Antioxidant Peptides from the Bactrian Camel Milk Hydrolysates".INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS 26.2(2020):641-650. |
入库方式： OAI收割
来源：新疆理化技术研究所
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