Functional Characterization of Dark Sleeper (Odontobutis obscura) TBK1 on IFN Regulation
文献类型：期刊论文
| 作者 | Chen, Jian2; Li, Zhuo Cong1,3; Lu, Long Feng1,3; Li, Pei2; Li, Xi-Yin1,3; Li, Shun1,3,4 |
| 刊名 | FRONTIERS IN IMMUNOLOGY
 |
| 出版日期 | 2019-05-03 |
| 卷号 | 10页码:11 |
| 关键词 | TBK1 IFN RLRs antiviral Odontobutis obscura |
| ISSN号 | 1664-3224 |
| DOI | 10.3389/fimmu.2019.00985 |
| 英文摘要 | In East Asia, the dark sleeper, Odontobutis obscura (O. obscura) is a crucial commercial species of freshwater fish; however, its molecular biology research is still undeveloped, including its innate immune system, which is pivotal to antiviral responses. In this study, we cloned and identified the characterization and kinase function of dark sleeper TANK-binding kinase 1 (TBK1), supplementing the evidence of the conservation of this classical factor in fish. First, the ORF of Odontobutis obscurus (O. obscura) TBK1 (OdTBK1) was cloned from liver tissue by RACE-PCR. Subsequent nucleic acid and amino acid sequence analysis suggested that OdTBK1 is homologous with other fish TBK1, and the N-terminal Serine/Threonine protein kinases catalytic domain (S_TKc) and C-terminal coiled coil domain (CCD) are conserved. Subsequently, the cellular distribution demonstrated that OdTBK1 was located in the cytoplasm region. With regard to the identification of functions, OdTBK1 activated several interferon (IFN) promoters' activity and induced downstream IFN-stimulated genes (ISGs) expression. In a canonical manner, wild-type OdTBK1 significantly phosphorylated interferon regulatory factor 3 (IRF3) but failed when the N-terminal region was truncated. Furthermore, overexpression of OdTBK1 decreased viral proliferation remarkably. Collectively, these data systematically analyzed the characterization and function of OdTBK1, initiating the study of the innate antiviral response of dark sleeper. |
| 资助项目 | National Natural Science Foundation of China[31502200] |
| WOS研究方向 | Immunology |
| 语种 | 英语 |
| WOS记录号 | WOS:000467335700002 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/7699]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Li, Shun |
| 作者单位 | 1.Univ Chinese Acad Sci, Wuhan, Hubei, Peoples R China 2.Wuhan Acad Agr Sci, Fisheries Res Inst, Wuhan, Hubei, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China 4.Minist Agr, Key Lab Aquaculture Dis Control, Wuhan, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Jian,Li, Zhuo Cong,Lu, Long Feng,et al. Functional Characterization of Dark Sleeper (Odontobutis obscura) TBK1 on IFN Regulation[J]. FRONTIERS IN IMMUNOLOGY,2019,10:11. |
| APA | Chen, Jian,Li, Zhuo Cong,Lu, Long Feng,Li, Pei,Li, Xi-Yin,&Li, Shun.(2019).Functional Characterization of Dark Sleeper (Odontobutis obscura) TBK1 on IFN Regulation.FRONTIERS IN IMMUNOLOGY,10,11. |
| MLA | Chen, Jian,et al."Functional Characterization of Dark Sleeper (Odontobutis obscura) TBK1 on IFN Regulation".FRONTIERS IN IMMUNOLOGY 10(2019):11. |
入库方式： OAI收割
来源：武汉植物园
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