Microcystin-LR Degradation and Gene Regulation of Microcystin-Degrading Novosphingobium sp. THN1 at Different Carbon Concentrations
文献类型：期刊论文
| 作者 | Wang, Juanping1,3,4; Wang, Chang1,3; Li, Qi3; Shen, Mengyuan1,3; Bai, Peng1,3; Li, Jionghui1,3; Lin, Yan3; Gan, Nanqin3; Li, Tao3; Zhao, Jindong2,3 |
| 刊名 | FRONTIERS IN MICROBIOLOGY
 |
| 出版日期 | 2019-08-06 |
| 卷号 | 10页码:14 |
| 关键词 | microcystin-LR biodegradation Novosphingobium sp. THN1 carbon availability gene expression transcriptome |
| ISSN号 | 1664-302X |
| DOI | 10.3389/fmicb.2019.01750 |
| 英文摘要 | The bacterium Novosphingobium sp. THN1 (THN1) is capable of degrading microcystin-LR (MC-LR). To study the ability of THN1 to degrade MC-LR and its possible mechanism(s) of regulation, we analyzed the effect of carbon concentrations on the degradation process. The MC-LR degradation rate peaked early and then declined during MC-LR biodegradation. Decreased levels of carbon in the medium caused the degradation peak to occur earlier. The expression of the functional gene mlrA, encoding a microcystinase, showed a similar trend to the MC-LR degradation rate at various carbon concentrations (r(2) = 0.717, p < 0.05), suggesting that regulation of mlrA expression may play an important role in MC-LR degradation by THN1. The total bacterial biomass decreased when the carbon source was limited and did not correlate with the MC-LR degradation rate. Transcriptomic analysis showed that MC-LR degradation differentially regulated 62.16% (2597/4178) of THN1 genes. A considerable number of differentially expressed genes (DEGs) during MC-LR degradation encoded proteins related to carbon-, nitrogen-, and amino acid-related pathways. At 2 h of MC-LR degradation, most DEGs (29/33) involved in carbon and nitrogen metabolism were downregulated. This indicated that MC-LR may regulate carbon and nitrogen pathways of Novosphingobium sp. THN1. KEGG pathway analysis indicated that the upregulated DEGs during MC-LR degradation were mainly related to amino acid degradation and substrate metabolism pathways. Particularly, we detected increased expression of glutathione metabolism-related genes from transcriptomic data at 2 h of MC-LR degradation compared with the gene expression of 0 h, such as GST family protein, glutathione peroxidase, S-(hydroxymethyl) glutathione dehydrogenase, and glutathione-dependent disulfide-bond oxidoreductase that have been reported to be involved in microcystin degradation. |
| 资助项目 | National Natural Science Foundation of China[91851118] ; Science and Technology Basic Resources Investigation Program of China[2017FY100300] ; Key Research Program of the Chinese Academy of Sciences[KFZD-SW-219] ; State Key Laboratory of Freshwater Ecology and Biotechnology[2019FBZ01] ; National Key R&D Program of China[2018YFA0903100] |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000478890900001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/8434]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Li, Tao |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Peking Univ, Coll Life Sci, State Key Lab Prot & Plant Genet Engn, Beijing, Peoples R China 3.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China 4.Wuhan Univ, Zhongnan Hosp, Sch Pharmaceut Sci, Key Lab Combinatorial Biosynth & Drug Discovery,M, Wuhan, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Juanping,Wang, Chang,Li, Qi,et al. Microcystin-LR Degradation and Gene Regulation of Microcystin-Degrading Novosphingobium sp. THN1 at Different Carbon Concentrations[J]. FRONTIERS IN MICROBIOLOGY,2019,10:14. |
| APA | Wang, Juanping.,Wang, Chang.,Li, Qi.,Shen, Mengyuan.,Bai, Peng.,...&Zhao, Jindong.(2019).Microcystin-LR Degradation and Gene Regulation of Microcystin-Degrading Novosphingobium sp. THN1 at Different Carbon Concentrations.FRONTIERS IN MICROBIOLOGY,10,14. |
| MLA | Wang, Juanping,et al."Microcystin-LR Degradation and Gene Regulation of Microcystin-Degrading Novosphingobium sp. THN1 at Different Carbon Concentrations".FRONTIERS IN MICROBIOLOGY 10(2019):14. |
入库方式： OAI收割
来源：武汉植物园
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