In Situ Capture of Chromatin Interactions by Biotinylated dCas9
文献类型：期刊论文
| 作者 | Liu, Xin1; Zhang, Yuannyu1; Li, Kailong1; Cao, Hui1; Ni, Min1; Liu, Yuxuan1; Gu, Zhimin1; Dickerson, Kathryn E.1; Xu, Jian1; Chen, Yong2 |
| 刊名 | CELL
 |
| 出版日期 | 2017 |
| 卷号 | 170期号:5页码:1028-+ |
| ISSN号 | 0092-8674 |
| 关键词 | Cell cycle cell cycle-regulated genes deep learning convolutional neural network machine learning classification |
| DOI | 10.1016/j.cell.2017.08.003 |
| 文献子类 | Article |
| 英文摘要 | Cis-regulatory elements (CREs) are commonly recognized by correlative chromatin features, yet the molecular composition of the vast majority of CREs in chromatin remains unknown. Here, we describe a CRISPR affinity purification in situ of regulatory elements (CAPTURE) approach to unbiasedly identify locus-specific chromatin-regulating protein complexes and long-range DNA interactions. Using an in vivo biotinylated nuclease-deficient Cas9 protein and sequence-specific guide RNAs, we show high-resolution and selective isolation of chromatin interactions at a single-copy genomic locus. Purification of human telomeres using CAPTURE identifies known and new telomeric factors. In situ capture of individual constituents of the enhancer cluster controlling human beta-globin genes establishes evidence for composition-based hierarchical organization. Furthermore, unbiased analysis of chromatin interactions at disease-associated cis-elements and developmentally regulated super-enhancers reveals spatial features that causally control gene transcription. Thus, comprehensive and unbiased analysis of locus-specific regulatory composition provides mechanistic insight into genome structure and function in development and disease. |
| 学科主题 | Biochemistry & Molecular Biology ; Cell Biology |
| WOS关键词 | LOCUS-CONTROL REGION ; BETA-GLOBIN LOCUS ; EMBRYONIC STEM-CELLS ; HUMAN GENOME ; CHROMOSOME CONFORMATION ; GENE-EXPRESSION ; TRANSGENIC MICE ; DNA INTERACTIONS ; TRANSCRIPTION ; PROTEINS |
| 语种 | 英语 |
| 出版者 | CELL PRESS |
| WOS记录号 | WOS:000408372400019 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/1142]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Univ Texas Southwestern Med Ctr Dallas, Dept Pediat, Childrens Med Ctr Res Inst, Dallas, TX 75390 USA; 2.Univ Texas Dallas, Dept Biol Sci, Ctr Syst Biol, Richardson, TX 75080 USA; 3.Tsinghua Univ, Dept Automat, Beijing 100084, Peoples R China, 4.Chinese Acad Sci, Shanghai Inst Biol Sci, CAS MPG Partner Inst Computat Biol, Key Lab Computat Biol, Shanghai 200031, Peoples R China; 5.Fudan Univ, Minister Educ, Key Lab Carcinogenesis & Canc Invas, Liver Canc Inst,Zhongshan Hosp, Shanghai 200032, Peoples R China; 6.Fudan Univ, Inst Biomed Sci, Shanghai 200032, Peoples R China; 7.Univ Texas Southwestern Med Ctr Dallas, Dept Obstet & Gynecol, Cecil H & Ida Green Ctr Reprod Biol Sci, Dallas, TX 75390 USA; 8.Tsinghua Univ, MOE Key Lab Bioinformat, Beijing 100084, Peoples R China; 9.Tsinghua Univ, Bioinformat Div, Beijing 100084, Peoples R China; 10.Tsinghua Univ, Ctr Synthet & Syst Biol, TNLIST, Beijing 100084, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Liu, Xin,Zhang, Yuannyu,Li, Kailong,et al. In Situ Capture of Chromatin Interactions by Biotinylated dCas9[J]. CELL,2017,170(5):1028-+. |
| APA | Liu, Xin.,Zhang, Yuannyu.,Li, Kailong.,Cao, Hui.,Ni, Min.,...&,.(2017).In Situ Capture of Chromatin Interactions by Biotinylated dCas9.CELL,170(5),1028-+. |
| MLA | Liu, Xin,et al."In Situ Capture of Chromatin Interactions by Biotinylated dCas9".CELL 170.5(2017):1028-+. |
入库方式： OAI收割
来源：上海营养与健康研究所
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