Phage-Display Based Discovery and Characterization of Peptide Ligands against WDR5
文献类型：期刊论文
| 作者 | Cao, Jiawen2,3; Fan, Tiantian2,3; Li, Yanlian2,3; Du, Zhiyan2,3; Chen, Lin2,3; Wang, Ying2,3; Wang, Xin2,3 ; Shen, Jingkang2,3; Huang, Xun1,3; Xiong, Bing2,3
|
| 刊名 | MOLECULES
 |
| 出版日期 | 2021-03-01 |
| 卷号 | 26期号:5页码:12 |
| 关键词 | phage display biopanning WDR5 cocrystal structure |
| DOI | 10.3390/molecules26051225 |
| 通讯作者 | Xiong, Bing(bxiong@simm.ac.cn) ; Cao, Danyan(caody@simm.ac.cn) |
| 英文摘要 | WD40 is a ubiquitous domain presented in at least 361 human proteins and acts as scaffold to form protein complexes. Among them, WDR5 protein is an important mediator in several protein complexes to exert its functions in histone modification and chromatin remodeling. Therefore, it was considered as a promising epigenetic target involving in anti-cancer drug development. In view of the protein-protein interaction nature of WDR5, we initialized a campaign to discover new peptide-mimic inhibitors of WDR5. In current study, we utilized the phage display technique and screened with a disulfide-based cyclic peptide phage library. Five rounds of biopanning were performed and isolated clones were sequenced. By analyzing the sequences, total five peptides were synthesized for binding assay. The four peptides are shown to have the moderate binding affinity. Finally, the detailed binding interactions were revealed by solving a WDR5-peptide cocrystal structure. |
| 资助项目 | National Natural Science Foundation of China[81773572] ; National Natural Science Foundation of China[91853126] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000628417600001 |
| 出版者 | MDPI |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/295667]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Xiong, Bing; Cao, Danyan |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Div Antitumor Pharmacol, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Dept Med Chem, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China 3.Univ Chinese Acad Sci, Dept Coll Pharm, 19A Yuquan Rd, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cao, Jiawen,Fan, Tiantian,Li, Yanlian,et al. Phage-Display Based Discovery and Characterization of Peptide Ligands against WDR5[J]. MOLECULES,2021,26(5):12. |
| APA | Cao, Jiawen.,Fan, Tiantian.,Li, Yanlian.,Du, Zhiyan.,Chen, Lin.,...&Cao, Danyan.(2021).Phage-Display Based Discovery and Characterization of Peptide Ligands against WDR5.MOLECULES,26(5),12. |
| MLA | Cao, Jiawen,et al."Phage-Display Based Discovery and Characterization of Peptide Ligands against WDR5".MOLECULES 26.5(2021):12. |
入库方式： OAI收割
来源：上海药物研究所
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