Exploring novel Cr(VI) remediation genes for Cr(VI)-contaminated industrial wastewater treatment by comparative metatranscriptomics and metagenomics
文献类型：期刊论文
| 作者 | Pei, Yaxin; Tao, Chen; Ling, Zhenmin; Yu, Zhengsheng; Ji, Jing; Khan, Aman; Mamtimin, Tursunay; Liu, Pu; Li, Xiangkai |
| 刊名 | SCIENCE OF THE TOTAL ENVIRONMENT
 |
| 出版日期 | 2020-11-10 |
| 卷号 | 742页码:1-8 |
| 关键词 | Novel genes Comparative metatranscriptomics High-efficiency Cr(VI) removal Industrial wastewater treatment Cr(VI) remediation characterization |
| ISSN号 | 0048-9697 |
| 英文摘要 | Microbial remediation is a promising method to treat Cr(VI) in industrial wastewater. The remediation efficiency and stress-resistance ability of Cr(V1) remediation genes in microbes are the limiting factors for their application in industrial wastewater treatment. To screen novel highly efficient Cr(Vl) remediation genes, comparative metatranscriptomic and metagenomic analyses were performed on long-term Cr(VI)-contaminated riparian soil with/without additional Cr(VI) treatment. The most suitable Cr(VI) treatment time was determined to be 30 min according to the high quality RNA yield and fold changes in gene expression. Six novel genes, which had complete open reading frames (OREs) in metagenomic libraries, were identified from unculturable microbes. In the phenotypic functional assay, all novel genes enhanced the Cr(VI) resistance/reduction ability of E. coll. In the industrial wastewater treatment, E-mcr and E-gsr presented at least 50% Cr(VI) removal efficiencies in the presence of 200-600 mu M of Cr(VI), without a decrease in efficiency over 17 days. The stress resistance assay showed that gsr increased the growth rate of E. coli by at least 30% under different extreme conditions, and thus, gsr was identified as a general stress-response gene. In the Cr valence distribution assay, E-mcr presented similar to 40 mu M higher extracellular Cr (III) compared to E-yieF. Additionally, transmission electron microscopy (TEM) of E-mcr showed bulk black agglomerates on the cell surface. Thus. mcr was identified as a membrane chromate reductase gene. This research provides a new idea for studying novel highly efficient contaminant remediation genes from unculturable microbes. (C) 2020 Elsevier B.V. All rights reserved. |
| 源URL | [http://ir.rcees.ac.cn/handle/311016/44682]  |
| 专题 | 生态环境研究中心_环境化学与生态毒理学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Pei, Yaxin,Tao, Chen,Ling, Zhenmin,et al. Exploring novel Cr(VI) remediation genes for Cr(VI)-contaminated industrial wastewater treatment by comparative metatranscriptomics and metagenomics[J]. SCIENCE OF THE TOTAL ENVIRONMENT,2020,742:1-8. |
| APA | Pei, Yaxin.,Tao, Chen.,Ling, Zhenmin.,Yu, Zhengsheng.,Ji, Jing.,...&Li, Xiangkai.(2020).Exploring novel Cr(VI) remediation genes for Cr(VI)-contaminated industrial wastewater treatment by comparative metatranscriptomics and metagenomics.SCIENCE OF THE TOTAL ENVIRONMENT,742,1-8. |
| MLA | Pei, Yaxin,et al."Exploring novel Cr(VI) remediation genes for Cr(VI)-contaminated industrial wastewater treatment by comparative metatranscriptomics and metagenomics".SCIENCE OF THE TOTAL ENVIRONMENT 742(2020):1-8. |
入库方式： OAI收割
来源：生态环境研究中心
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