Cathepsin B of Cynoglossus semilaevis: Identification, expression, and activity analysis
文献类型：期刊论文
| 作者 | Chen, Ling1,2; Sun, Li1 |
| 刊名 | COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
 |
| 出版日期 | 2012 |
| 卷号 | 161期号:1页码:54-59 |
| 关键词 | Cathepsin B Teleost Fish Cynoglossus semilaevis Cysteine protease |
| ISSN号 | 1096-4959 |
| 通讯作者 | Sun, L (reprint author), Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, 7 Nanhai Rd, Qingdao 266071, Peoples R China. |
| 英文摘要 | Cathepsin B (EC 3.4.22.1) is a member of the papain family cysteine protease and in mammals is known to be involved in protein degradation and other biological functions. However, very little is known about the function of cathepsin B in fish. In this study, we identified and analyzed a cathepsin B homologue (CsCatB) from tongue sole (Cynoglossus semilaevis, Pleuronectiformes), an economic fish species cultured in China. CsCatB is composed of 322 amino acid residues and shares 70-81.3% overall sequence identities with its counterpart in teleosts and humans. CsCatB possesses typical cathepsin B structural features including the propeptide region and the papain family cysteine protease domain, the latter containing the four catalytic residues (Q101, C107, H277, and N297) that are conserved in lower and higher vertebrates. Quantitative real time RT-PCR analysis showed that CsCatB expression occurred in multiple tissues and was positively regulated by bacterial infection and by immunization with a subunit vaccine. Recombinant CsCatB purified from Escherichia colt exhibited apparent protease activity, which was optimal at 35 degrees C and pH 5.5. In contrast, a mutant CsCatB bearing glutamic acid substitution at H277 was dramatically reduced in proteolytic activity. These results indicate that CsCatB is a biologically active protease that is likely to be involved in host immune response during bacterial infection and vaccination. (C) 2011 Elsevier Inc. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology ; Zoology |
| 收录类别 | SCI |
| 原文出处 | 10.1016/j.cbpb.2011.09.008 |
| 语种 | 英语 |
| WOS记录号 | WOS:000297833700006 |
| 公开日期 | 2013-09-24 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/12292]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Ling,Sun, Li. Cathepsin B of Cynoglossus semilaevis: Identification, expression, and activity analysis[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2012,161(1):54-59. |
| APA | Chen, Ling,&Sun, Li.(2012).Cathepsin B of Cynoglossus semilaevis: Identification, expression, and activity analysis.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,161(1),54-59. |
| MLA | Chen, Ling,et al."Cathepsin B of Cynoglossus semilaevis: Identification, expression, and activity analysis".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 161.1(2012):54-59. |
入库方式： OAI收割
来源：海洋研究所
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