Loss of E-cadherin due to road dust PM2.5 activates the EGFR in human pharyngeal epithelial cells
文献类型：期刊论文
| 作者 | Nguyen Thanh Tung1,2; Ho, Kin-Fai3; Niu, Xinyi4; Sun, Jian5; Shen, Zhenxing5; Wu, Feng6,7; Cao, Junji6,7; Hoang Ba Dung2; Tran Phan Chung Thuy8; Hsiao, Ta-Chih9 |
| 刊名 | ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
 |
| 出版日期 | 2021-05-26 |
| 页码 | 16 |
| ISSN号 | 0944-1344 |
| 关键词 | Air pollution Allergy Chemical Metal Particulate matter Upper airway |
| DOI | 10.1007/s11356-021-14469-2 |
| 通讯作者 | Liu, Wen-Te(lion5835@gmail.com) ; Chuang, Hsiao-Chi(chuanghc@tmu.edu.tw) |
| 英文摘要 | Exposure to road dust particulate matter (PM) causes adverse health impacts on the human airway. However, the effects of road dust on the upper airway epithelium in humans remain unclear. We investigated the involvement of the epidermal growth factor receptor (EGFR) after PM with an aerodynamic diameter of < 2.5 mu m (PM2.5)-induced E-cadherin disruption of human pharyngeal epithelial cells. First, we collected road dust PM2.5 from 10 Chinese cities, including Wuhan, Nanjing, Shanghai, Guangzhou, Chengdu, Beijing, Lanzhou, Tianjin, Harbin, and Xi'an. Human pharyngeal FaDu cells were exposed to road dust PM2.5 at 50 mu g/mL for 24 h, cytotoxicity (cell viability and lactate dehydrogenase (LDH)) was assessed, and expressions of the proinflammatory interleukin (IL)-6 and high-mobility group box 1 (HMGB1) protein, receptor for advanced glycation end products (RAGE), occludin, E-cadherin, EGFR, and phosphorylated (p)-EGFR were determined. The E-cadherin gene was then knocked down to investigate EGFR activation in FaDu cells. Exposure to road dust PM2.5 resulted in a decrease in cell viability and increases in LDH and IL-6. Our data suggested that PM2.5 could decrease expressions of occludin and E-cadherin and increase expressions of EGFR and p-EGFR, which was confirmed by E-cadherin-knockdown. Our results showed a negative association between the alterations in E-cadherin and total elemental components in correlation analysis, especially S, Cl, K, Ti, Mn, Fe, Cu, Zn, and Pb. Exposure to metals in PM2.5 from road dust may lead to loss of the barrier function of the upper airway epithelium and activation of the EGFR. Our study showed the adverse effects of road dust PM2.5 on pharyngeal epithelial cells of the human upper airway. |
| WOS关键词 | FINE PARTICULATE MATTER ; GROWTH-FACTOR RECEPTOR ; SOURCE PROFILES ; OXIDATIVE STRESS ; AIR-POLLUTION ; FUGITIVE DUST ; SOURCE APPORTIONMENT ; ALLERGIC RHINITIS ; MESENCHYMAL TRANSITION ; ELEMENTAL CARBON |
| 资助项目 | Ministry of Science and Technology of Taiwan[109-2314-B-038-093-MY3] |
| WOS研究方向 | Environmental Sciences & Ecology |
| 语种 | 英语 |
| 出版者 | SPRINGER HEIDELBERG |
| WOS记录号 | WOS:000654150700004 |
| 资助机构 | Ministry of Science and Technology of Taiwan |
| 源URL | [http://ir.ieecas.cn/handle/361006/16705]  |
| 专题 | 地球环境研究所_粉尘与环境研究室 |
| 通讯作者 | Liu, Wen-Te; Chuang, Hsiao-Chi |
| 作者单位 | 1.Taipei Med Univ, Coll Med, Int PhD Program Med, Taipei, Taiwan 2.Cho Ray Hosp, Otorhinolaryngol Dept, Ho Chi Minh City, Vietnam 3.Chinese Univ Hong Kong, JC Sch Publ Hlth & Primary Care, Hong Kong, Peoples R China 4.Xi An Jiao Tong Univ, Sch Human Settlements & Civil Engn, Xian, Peoples R China 5.Xi An Jiao Tong Univ, Dept Environm Sci & Engn, Xian, Peoples R China 6.Chinese Acad Sci, Inst Earth Environm, Key Lab Aerosol Chem & Phys, Xian, Peoples R China 7.Chinese Acad Sci, Inst Earth Environm, SKLLQG, Xian, Peoples R China 8.Vietnam Natl Univ Ho Chi Minh City, Fac Med, Otorhinolaryngol Dept, Ho Chi Minh City, Vietnam 9.Natl Taiwan Univ, Grad Inst Environm Engn, Taipei, Taiwan 10.Taipei Med Univ, Shuang Ho Hosp, Dept Internal Med, Div Pulm Med, New Taipei, Taiwan |
| 推荐引用方式 GB/T 7714 | Nguyen Thanh Tung,Ho, Kin-Fai,Niu, Xinyi,et al. Loss of E-cadherin due to road dust PM2.5 activates the EGFR in human pharyngeal epithelial cells[J]. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,2021:16. |
| APA | Nguyen Thanh Tung.,Ho, Kin-Fai.,Niu, Xinyi.,Sun, Jian.,Shen, Zhenxing.,...&Chuang, Hsiao-Chi.(2021).Loss of E-cadherin due to road dust PM2.5 activates the EGFR in human pharyngeal epithelial cells.ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH,16. |
| MLA | Nguyen Thanh Tung,et al."Loss of E-cadherin due to road dust PM2.5 activates the EGFR in human pharyngeal epithelial cells".ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2021):16. |
入库方式： OAI收割
来源：地球环境研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。