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MicroRNA-16-5p regulates cell survival, cell cycle and apoptosis by targetingAKT3in prostate cancer cells

文献类型：期刊论文

作者Wang, Fang2,4,5; Wang, Wendi3; Lu, Lina1; Xie, Yi4,5; Yan, Junfang2,4,5; Chen, Yuhong2,4,5; Di, Cuixia4,5; Gan, Lu2,4,5; Si, Jing4,5; Zhang, Hong4,5
刊名ONCOLOGY REPORTS
出版日期2020-09-01
卷号44期号:3页码:1282-1292
ISSN号1021-335X
关键词microRNA-16-5p cell survival cell cycle apoptosis prostate cancer
DOI10.3892/or.2020.7682
通讯作者Zhang, Hong(zhangh@impcas.ac.cn) ; Mao, Aihong(maoaih@impcas.ac.cn)
英文摘要Prostate cancer (PCa) is a malignancy with the highest morbidity rate in 105 countries worldwide and was a major cause of cancer-associated death in men in 2018. Accumulating evidence suggests that microRNAs (miRNAs/miRs) have important functions in the carcinogenesis of PCa, and may provide novel treatment targets. Previous studies have indicated that miR-16-5p is associated with PCa. However, the relevance and importance of miR-16-5p in PCa carcinogenesis are still not completely understood. In the current study, we aimed to investigate the role and mechanism of miR-16-5p in PCa carcinogenesis. The results showed that miR-16-5p was markedly downregulated in PCa cells, and MTS assay, colony formation, flow cytometric analyses demonstrated that miR-16-5p inhibited PCa cell survival, regulated cell cycle distribution and induced apoptosis. Moreover, luciferase reporter assay and western blot analysis showed that miR-16-5p directly targetsAKT3(AKT serine/threonine kinase 3), which is associated with PCa carcinogenesis, and the effects of the downregulation of AKT3 were similar to the effects of upregulation of miR-16-5p in PC-3 cells. In conclusion, our data clarify that miR-16-5p has anticancer functions in PCa cells, and our findings provide experimental evidence to highlight the potential value of miR-targeting treatment strategies for PCa.
WOS关键词PROLIFERATION ; EXPRESSION ; RNA ; METASTASIS ; BIOGENESIS ; MANAGEMENT ; PATHWAY ; 13Q14 ; AKT3
资助项目National Key R&D Program of China[2018YFE0205100] ; National Natural Science Foundation of China[11665003] ; Cancer Research Youth Science Foundation of Chinese Anti-Cancer Association[CAYC18A06]
WOS研究方向Oncology
语种英语
出版者SPANDIDOS PUBL LTD
WOS记录号WOS:000563563700041
资助机构National Key R&D Program of China ; National Natural Science Foundation of China ; Cancer Research Youth Science Foundation of Chinese Anti-Cancer Association
源URL[http://119.78.100.186/handle/113462/139859]  
专题中国科学院近代物理研究所
通讯作者Zhang, Hong; Mao, Aihong
作者单位1.Northwest Minzu Univ, Sch Chem Engn, Lanzhou 730030, Gansu, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
3.Gansu Prov Matern & Child Care Hosp, Inst Res Ctr, Lanzhou 730050, Gansu, Peoples R China
4.Chinese Acad Sci, Inst Modern Phys, 509 Nanchang Rd, Lanzhou 730000, Gansu, Peoples R China
5.Chinese Acad Sci, Key Lab Heavy Ion Radiat Med, Lanzhou 730000, Gansu, Peoples R China
6.Gansu Prov Acad Inst Med Res, 2 Xiaoxihu East St, Lanzhou 730050, Gansu, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Fang,Wang, Wendi,Lu, Lina,et al. MicroRNA-16-5p regulates cell survival, cell cycle and apoptosis by targetingAKT3in prostate cancer cells[J]. ONCOLOGY REPORTS,2020,44(3):1282-1292.
APA Wang, Fang.,Wang, Wendi.,Lu, Lina.,Xie, Yi.,Yan, Junfang.,...&Mao, Aihong.(2020).MicroRNA-16-5p regulates cell survival, cell cycle and apoptosis by targetingAKT3in prostate cancer cells.ONCOLOGY REPORTS,44(3),1282-1292.
MLA Wang, Fang,et al."MicroRNA-16-5p regulates cell survival, cell cycle and apoptosis by targetingAKT3in prostate cancer cells".ONCOLOGY REPORTS 44.3(2020):1282-1292.

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来源：近代物理研究所

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