ANTP-SMACN7 fusion peptide alone induced high linear energy transfer irradiation radiosensitization in non-small cell lung cancer cell lines
文献类型：期刊论文
| 作者 | Xie, Yi1,5 ; Wang, Bing4; Du, Liqing3; Wang, Yan3; Xu, Chang3; Zhang, Hong1,5; Wen, Kaixue2; Liu, Qiang3; Katsube, Takanori4
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| 刊名 | CANCER BIOLOGY & MEDICINE
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| 出版日期 | 2021-01-12 |
| 页码 | 12 |
| ISSN号 | 2095-3941 |
| 关键词 | Fe-particle radiation carbon-particle radiation non-small cell lung cancer cells caspase radiosensitizer |
| DOI | 10.20892/j.issn.2095-3941.2020.0569 |
| 通讯作者 | Liu, Qiang(liuqiang@irm-cams.ac.cn) ; Katsube, Takanori(katsube.takanori@qst.go.jp) |
| 英文摘要 | Objective: The aim of the present study was to investigate the mechanisms responsible for the radiation-sensitizing effect of antennapedia proteins, ANTP-SMACN7, on lung cancer cells treated with accelerated carbon and Fe particle irradiation. Methods: The ANTP-SMACN7 fusion peptide was synthesized and linked to fluorescein isothiocyanate to determine its ability to penetrate cells. A549 and NCI-H460 cells, human non-small cell lung cancer (NSCLC) cell lines, were irradiated with X-ray or high linear energy transfer (LET) irradiation with or without ANTP-SMACN7 treatment. Cellular survival, apoptosis, and protein expression were studied by colony formation assays, flow cytometry, and western blot analyses, respectively. Results: ANTP-SMACN7 fusion proteins entered the cells and promoted A549 and NCI-H460 cell high LET irradiation radiosensitization. High LET irradiation was more efficient for clonogenic cell killing and the induction of apoptosis (P < 0.05). Treatment with ANTP-SMACN7 significantly reduced the A549 and NCI-H460 cell clone-forming percentages and increased apoptosis through inhibition of the X-linked inhibitor of apoptosis protein and the activation of caspase-3 and caspase-9. Conclusions: Regarding pharmaceutical radiosensitization, these findings provided a way to improve high-LET clinical radiotherapy for NSCLC patients. |
| WOS关键词 | X-LINKED INHIBITOR ; APOPTOSIS PROTEIN ; ANTENNAPEDIA HOMEODOMAIN ; 3RD HELIX ; SENSITIVITY ; RESISTANCE |
| 资助项目 | National Key R&D Program of China[2018YFE0205100] ; National Key R&D Program of China[2018YFE0205101] ; National Natural Science Foundation of China[11605260] ; National Natural Science Foundation of China[31670859] ; Key Research and Development Projects of Shanxi Province[201903D321115] ; Science and Technology Talent Project in Lanzhou[2018-RC-66] ; Science and Technology Project of Chengguan District of Lanzhou[2020RCCX0038] ; CAMS Innovation Fund for Medical Science[2017-I2M-1-016] ; Ministry of Education, Culture, Sports, and Science Technology[JP15K21745] ; Ministry of Education, Culture, Sports, and Science Technology[15H05944] ; Ministry of Education, Culture, Sports, and Science Technology[15H05935] ; Ministry of Education, Culture, Sports, and Science Technology[14J313] |
| WOS研究方向 | Oncology ; Research & Experimental Medicine |
| 语种 | 英语 |
| 出版者 | CHINA ANTI-CANCER ASSOC |
| WOS记录号 | WOS:000729392000001 |
| 资助机构 | National Key R&D Program of China ; National Natural Science Foundation of China ; Key Research and Development Projects of Shanxi Province ; Science and Technology Talent Project in Lanzhou ; Science and Technology Project of Chengguan District of Lanzhou ; CAMS Innovation Fund for Medical Science ; Ministry of Education, Culture, Sports, and Science Technology |
| 源URL | [http://119.78.100.186/handle/113462/140129]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Liu, Qiang; Katsube, Takanori |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, Lanzhou 730000, Peoples R China 2.Shanxi Acad Med Sci, Shanxi Bethune Hosp, Taiyuan 030031, Peoples R China 3.Chinese Acad Med Sci & Peking Union Med Coll, Inst Radiat Med, Tianjin 300192, Peoples R China 4.Natl Inst Quantum & Radiol Sci & Technol, Natl Inst Radiol Sci, Chiba 2638555, Japan 5.Adv Energy Sci & Technol Guangdong Lab, Huizhou 516029, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xie, Yi,Wang, Bing,Du, Liqing,et al. ANTP-SMACN7 fusion peptide alone induced high linear energy transfer irradiation radiosensitization in non-small cell lung cancer cell lines[J]. CANCER BIOLOGY & MEDICINE,2021:12. |
| APA | Xie, Yi.,Wang, Bing.,Du, Liqing.,Wang, Yan.,Xu, Chang.,...&Katsube, Takanori.(2021).ANTP-SMACN7 fusion peptide alone induced high linear energy transfer irradiation radiosensitization in non-small cell lung cancer cell lines.CANCER BIOLOGY & MEDICINE,12. |
| MLA | Xie, Yi,et al."ANTP-SMACN7 fusion peptide alone induced high linear energy transfer irradiation radiosensitization in non-small cell lung cancer cell lines".CANCER BIOLOGY & MEDICINE (2021):12. |
入库方式： OAI收割
来源：近代物理研究所
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