Inhibition of Wnt signalling pathway by XAV939 enhances radiosensitivity in human cervical cancer HeLa cells
文献类型：期刊论文
| 作者 | Zhang, Jinhua1,2,3; Si, Jing1,2,3 ; Gan, Lu1,2,3; Guo, Menghuan4; Yan, Junfang1,2,3; Chen, Yuhong1,2,3; Wang, Fang1,2,3; Xie, Yi1,2,3; Wang, Yupei1,2,3; Zhang, Hong1,2,3
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| 刊名 | ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
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| 出版日期 | 2020 |
| 卷号 | 48期号:1页码:479-487 |
| ISSN号 | 2169-1401 |
| 关键词 | C-12(6+) irradiation cervical carcinoma Wnt beta-catenin pathway XAV939 radio-sensitivity |
| DOI | 10.1080/21691401.2020.1716779 |
| 通讯作者 | Si, Jing(sijing@impcas.ac.cn) ; Zhang, Hong(zhangh@impcas.ac.cn) |
| 英文摘要 | Cervical cancer is the second most common malignant tumour threatening women's health. In recent years, heavy-ion beam therapy is becoming a newly emerging therapeutic mean of cancer; however, radio-resistance and radiation-induced damage constitute the main obstacles for curative treatment of cervical cancer. Therefore, to identify the radiosensitizers is essential. Here, we investigated the effects of Wnt signalling pathway on the response of C-12(6+) radiation in HeLa cells. XAV939, an inhibitor of Wnt signalling pathway, was added two hours before C-12(6+) radiation.C-12(6+) radiation inhibited the viability of HeLa cells in a time-dependent manner, and inhibiting Wnt signalling using XAV939 significantly intensified this stress. Meanwhile, C-12(6+) radiation induced a significant increased cell apoptosis, G2/M phase arrest, and the number of gamma-H2AX foci. Supplementation with XAV939 significantly increased the effects induced by C-12(6+) radiation alone. Combining XAV939 with C-12(6+) irradiation, the expression of apoptotic genes (p53, Bax, Bcl-2) was significantly increased, while the expression of Wnt-related genes (Wnt3a, Wnt5a, beta-catenin, cyclin D1 and c-Myc) was significantly decreased. Overall, these findings suggested that blockage of the Wnt/beta-catenin pathway effectively sensitizes HeLa cells to C-12(6+) irradiation, and it may be a potential therapeutic approach in terms of increasing the clinical efficacy of C-12(6+) beams. |
| WOS关键词 | CARBON-ION RADIOTHERAPY ; WNT/BETA-CATENIN PATHWAY ; DIFFERENTIATION ; IRRADIATION ; ACTIVATION ; CARCINOMA ; RADIATION ; AUTOPHAGY ; IMPACT |
| 资助项目 | Ministry of Science and Technology National Key RD Project[2018YFE0205100] ; Key Program of the National Natural Science Foundation of China[U1632270] ; National Natural Science Foundation of China[11675234] ; National Natural Science Foundation of China[11605255] ; National Natural Science Foundation of China[11875061] ; Science and Technology Plan Project of Chengguan district, Lanzhou[2019RCCX0071] ; Lanzhou Talent Innovation and Entrepreneurship Project[2019-RC76] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Engineering ; Materials Science |
| 语种 | 英语 |
| 出版者 | TAYLOR & FRANCIS LTD |
| WOS记录号 | WOS:000509033500001 |
| 资助机构 | Ministry of Science and Technology National Key RD Project ; Key Program of the National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science and Technology Plan Project of Chengguan district, Lanzhou ; Lanzhou Talent Innovation and Entrepreneurship Project |
| 源URL | [http://119.78.100.186/handle/113462/141455]  |
| 专题 | 中国科学院近代物理研究所 |
| 通讯作者 | Si, Jing; Zhang, Hong |
| 作者单位 | 1.Chinese Acad Sci, Inst Modern Phys, Dept Radiat Med, Lanzhou, Peoples R China 2.Chinese Acad Sci, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou, Peoples R China 3.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China 4.Lanzhou Univ, Sch Pharm, Lanzhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Jinhua,Si, Jing,Gan, Lu,et al. Inhibition of Wnt signalling pathway by XAV939 enhances radiosensitivity in human cervical cancer HeLa cells[J]. ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY,2020,48(1):479-487. |
| APA | Zhang, Jinhua.,Si, Jing.,Gan, Lu.,Guo, Menghuan.,Yan, Junfang.,...&Zhang, Hong.(2020).Inhibition of Wnt signalling pathway by XAV939 enhances radiosensitivity in human cervical cancer HeLa cells.ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY,48(1),479-487. |
| MLA | Zhang, Jinhua,et al."Inhibition of Wnt signalling pathway by XAV939 enhances radiosensitivity in human cervical cancer HeLa cells".ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY 48.1(2020):479-487. |
入库方式： OAI收割
来源：近代物理研究所
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