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Mitochondrial proteomics reveals the mechanism of spermatogenic cells apoptosis induced by carbon ion radiation in zebrafish

文献类型：期刊论文

作者Li, Hongyan1,2,3,4; Zhang, Weihong5; Zhang, Hong1,2,3,4,6; Xie, Yi1,2,3; Sun, Chao1,2,3; Di, Cuixia1,2,3; Si, Jing1,2,3; Gan, Lu1,2,3; Yan, Junfang1,2,3,4
刊名JOURNAL OF CELLULAR PHYSIOLOGY
出版日期2019-12-01
卷号234期号:12页码:22439-22449
关键词carbon ion radiation mitochondria spermatogenic cells zebrafish
ISSN号0021-9541
DOI10.1002/jcp.28808
通讯作者Zhang, Hong(zhangh@impcas.ac.cn)
英文摘要The mitochondrial proteins involved in spermatogenic cells apoptosis in zebrafish after carbon ion radiation (CIR) were screened. The relative biological effectiveness (RBE) of CIR in zebrafish testes was investigated. Apoptosis of testicular cells was measured within 24hr following 1 and 4Gy CIR. Immunoblotting was used to assess the levels of mitochondrial apoptotic proteins in testes, and proliferative and apoptotic spermatogenic cells were detected by immunofluorescence after CIR. Label-free quantitative(LFQ) and parallel reaction monitoring-based target proteomics (PRM) were combined to screen and validate differential mitochondrial proteins in testes between 4Gy and control groups at 24hr after CIR. The RBE of CIR in zebrafish testes was 1.48 +/- 0.04, and induction of apoptosis by CIR was higher than that of X-rays in testicular cells. Mitochondrial apoptotic pathways play a crucial role in spermatogenic cells apoptosis after CIR, with 60 differential mitochondrial proteins identified. Among 20 target proteins, 12 were significantly upregulated, 2 were significantly downregulated in the 4Gy CIR group. The results of PRM were consistent with label-free analysis. This is the first study to screen the differential mitochondrial proteins and provide useful information to understand the underlying mechanisms of spermatogenic cell apoptosis in zebrafish following CIR.
WOS关键词LOW-DOSE RADIATION ; MOUSE TESTES ; IRRADIATION ; DEATH ; MODULATION ; AUTOPHAGY ; BEAMS
资助项目Ministry of Science and Technology National Key RD project[2016YFC0904600] ; Ministry of Science and Technology National Key RD project[SQ2018YFE020524] ; Key Program of National Natural Science Foundation of China[U1432248] ; National Natural Science Foundation of China[11505244] ; National Natural Science Foundation of China[11875061] ; Western Talent Program of the Chinese Academy of Sciences[Y560020XB0]
WOS研究方向Cell Biology ; Physiology
语种英语
WOS记录号WOS:000484376600085
出版者WILEY
资助机构Ministry of Science and Technology National Key RD project ; Key Program of National Natural Science Foundation of China ; National Natural Science Foundation of China ; Western Talent Program of the Chinese Academy of Sciences
源URL[http://119.78.100.186/handle/113462/141767]  
专题中国科学院近代物理研究所
通讯作者Zhang, Hong
作者单位1.Chinese Acad Sci, Inst Modern Phys, Dept Radiat Med, Lanzhou 730000, Gansu, Peoples R China
2.Chinese Acad Sci, Inst Modern Phys, Key Lab Heavy Ion Radiat Biol & Med, Lanzhou, Gansu, Peoples R China
3.Chinese Acad Sci, Inst Modern Phys, Key Lab Basic Res Heavy Ion Radiat Applicat Med, Lanzhou, Gansu, Peoples R China
4.Univ Chinese Acad Sci, Sch Nucl Sci & Technol, Beijing, Peoples R China
5.Gansu Wuwei Tumor Hosp, Dept Ultrasonog, Wuwei, Peoples R China
6.Gansu Wuwei Tumor Hosp, Dept Sci & Technol, Wuwei, Peoples R China
推荐引用方式
GB/T 7714		 Li, Hongyan,Zhang, Weihong,Zhang, Hong,et al. Mitochondrial proteomics reveals the mechanism of spermatogenic cells apoptosis induced by carbon ion radiation in zebrafish[J]. JOURNAL OF CELLULAR PHYSIOLOGY,2019,234(12):22439-22449.
APA Li, Hongyan.,Zhang, Weihong.,Zhang, Hong.,Xie, Yi.,Sun, Chao.,...&Yan, Junfang.(2019).Mitochondrial proteomics reveals the mechanism of spermatogenic cells apoptosis induced by carbon ion radiation in zebrafish.JOURNAL OF CELLULAR PHYSIOLOGY,234(12),22439-22449.
MLA Li, Hongyan,et al."Mitochondrial proteomics reveals the mechanism of spermatogenic cells apoptosis induced by carbon ion radiation in zebrafish".JOURNAL OF CELLULAR PHYSIOLOGY 234.12(2019):22439-22449.

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来源：近代物理研究所

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