Imaging and measuring the rituximab-induced changes of mechanical properties in B-lymphoma cells using atomic force microscopy
文献类型：期刊论文
| 作者 | Li M(李密) ; Liu LQ(刘连庆); Wang YC(王越超); Dong ZL(董再励); Tabata, Osamu; Xiao XB(肖秀斌); Zhang WJ(张伟京)
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| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
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| 出版日期 | 2011 |
| 卷号 | 404期号:2页码:689-694 |
| 关键词 | Atomic Force Microscopy Mechanical Properties Rituximab Young's Modulus |
| ISSN号 | 0006-291X |
| 产权排序 | 1 |
| 英文摘要 | The topography and mechanical properties of single B-lymphoma cells have been investigated by atomic force microscopy (AFM). With the assistance of microfabricated patterned pillars, the surface topography and ultrastructure of single living B-lymphoma cell were visualized by AFM. The apoptosis of B-lymphoma cells induced by rituximab alone was observed by acridine orange/ethidium bromide (AO/EB) double fluorescent staining. The rituximab-induced changes of mechanical properties in B-lymphoma cells were measured dynamically and the results showed that B-lymphoma cells became dramatically softer after incubation with rituximab. These results can improve our understanding of rituximab effect and will facilitate the further investigation of the underlying mechanisms. |
| WOS关键词 | ANTI-CD20 MONOCLONAL-ANTIBODIES ; LIVING CELLS ; LIPID RAFTS ; MICROBIAL-CELLS ; APOPTOSIS ; FIBROBLASTS ; CD20 ; CYTOTOXICITY ; CYTOSKELETON ; RESOLUTION |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000286543800020 |
| 资助机构 | This work was supported by National Natural Science Foundation of China (60904095, 60635040), National High Technology Research and Development Program of China (2009AA03Z316) and the CAS FEA International Partnership Program for Creative Research Teams. |
| 公开日期 | 2012-05-29 |
| 源URL | [http://ir.sia.cn/handle/173321/7364]  |
| 专题 | 沈阳自动化研究所_机器人学研究室 |
| 通讯作者 | Liu LQ(刘连庆); Wang YC(王越超) |
| 作者单位 | 1.Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences, Beijing 100071, China 2.Graduate University of Chinese Academy of Sciences, Beijing 100049, China 3.Department of Electrical and Computer Engineering, Michigan State University, East Lansing, MI 48824, USA 4.Department of Micro Engineering, Kyoto University, Kyoto 606-8501, Japan 5.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China |
| 推荐引用方式 GB/T 7714 | Li M,Liu LQ,Wang YC,et al. Imaging and measuring the rituximab-induced changes of mechanical properties in B-lymphoma cells using atomic force microscopy[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2011,404(2):689-694. |
| APA | Li M.,Liu LQ.,Wang YC.,Dong ZL.,Tabata, Osamu.,...&Zhang WJ.(2011).Imaging and measuring the rituximab-induced changes of mechanical properties in B-lymphoma cells using atomic force microscopy.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,404(2),689-694. |
| MLA | Li M,et al."Imaging and measuring the rituximab-induced changes of mechanical properties in B-lymphoma cells using atomic force microscopy".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 404.2(2011):689-694. |
入库方式： OAI收割
来源：沈阳自动化研究所
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