Drug-Induced Changes of Topography in Living B Lymphoma Cells: An Atomic Force Microscopy Study
文献类型：会议论文
| 作者 | Li M(李密) ; Liu LQ(刘连庆); Wang YC(王越超); Dong ZL(董再励); Yoshikazu Hirai; Osamu Tabata; Xiao XB(肖秀斌); Zhang WJ(张伟京)
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| 出版日期 | 2011 |
| 会议日期 | August 29 - September 2, 2011 |
| 会议地点 | Changchun, China |
| 关键词 | Atomic Force Microscopy b Lymphoma Rituximab |
| 页码 | 4 pp. |
| 英文摘要 | Due to the particular imaging mode, atomic force microscopy (AFM) provides a new technology for characterizing the topography structures of individual living cells under nearphysiological conditions. However, owing to the lack of adequate immobilization method, using AFM to visualize the topography of mammalian suspension cells is still a big challenge. In this paper, a method for imaging living mammalian suspension cells by AFM was presented. In this method, single B lymphoma suspension cells were mechanically trapped by microfabricated pillars which were coated with positively charged poly-L-lysine. Based on this immobilization, the Rituximab-induced topography changes of individual B lymphoma cells were visualized. The roughness of the cell surface’s local area before and after the stimulation of Rituximab was analysed. The force curves obtained on the cell surface before and after adding Rituximab were also analysed. The experimental results indicate the efficacy of the pillar-based immobilization and will facilitate the research of the physiological activities of living mammalian suspension cells. |
| 源文献作者 | National Natural Science Foundation of China |
| 产权排序 | 1 |
| 会议录 | The First International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale
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| 语种 | 英语 |
| 源URL | [http://ir.sia.cn/handle/173321/8677]  |
| 专题 | 沈阳自动化研究所_机器人学研究室 |
| 通讯作者 | Liu LQ(刘连庆); Wang YC(王越超) |
| 作者单位 | 1.Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences, Beijing, China 2.State Key Laboratory of Robotics, Shenyang Institute of Automation Chinese Academy of Sciences, Shenyang, China 3.Gruduate School of Chinese Academy of Sciences, Beijing, China 4.Department of Micro Engineering, Kyoto University, Kyoto, Japan 5.Department of Electrical and Computer Engineering, Michigan State University, Michigan, USA |
| 推荐引用方式 GB/T 7714 | Li M,Liu LQ,Wang YC,et al. Drug-Induced Changes of Topography in Living B Lymphoma Cells: An Atomic Force Microscopy Study[C]. 见:. Changchun, China. August 29 - September 2, 2011. |
入库方式： OAI收割
来源：沈阳自动化研究所
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