Imaging and measuring the protein distribution of lymphoma cells using atomic force microscopy
文献类型：会议论文
| 作者 | Li M(李密) ; Liu LQ(刘连庆); Wang YC(王越超); Dong ZL(董再励); Li GY(李广勇); Tabata, Osamu; Xiao XB(肖秀斌); Zhang WJ(张伟京)
|
| 出版日期 | 2011 |
| 会议日期 | February 20-23, 2011 |
| 会议地点 | Kaohsiung, Taiwan |
| 关键词 | Cell Immobilization Cell Membranes Cytology Mammals Microfabrication Monoclonal Antibodies Oncology |
| 页码 | 188-191 |
| 英文摘要 | Atomic force microscopy (AFM) has proven to be a powerful tool for imaging the topographic properties of biological samples and measuring the molecular specific binding forces at cellular and molecular levels. However, there are still great difficulties in using AFM to acquire images of mammalian suspension live cells due to the suspension growth manner and the softness of the cell membrane. In this paper, lymphoma live cells were trapped in microfabricated patterned pillars and then imaged by AFM in phosphate buffered saline (PBS) solution. In addition, the CD20 distribution on the lymphoma cell surface was visualized three-dimensionally by tethering rituximabs (anti-CD20 monoclonal antibody) onto the AFM tips. The experimental results indicate that immobilization with microfabricated patterned pillars provides a novel way for anchoring suspension mammalian cells, opening the way for further investigation of the molecular mechanisms of rituximab's anti-cancer effect. © 2011 IEEE. |
| 源文献作者 | Institute of Electrical and Electronics Engineers (IEEE); IEEE Nanotechnology Council (NTC); National Cheng Kung University; National Tsing Hua University; Chinese International NEMS Socity (CINS) |
| 产权排序 | 1 |
| 会议录 | NEMS 2011 - 6th IEEE International Conference on Nano/Micro Engineered and Molecular Systems
 |
| 会议录出版者 | IEEE Computer Society |
| 会议录出版地 | Piscataway, NJ |
| 语种 | 英语 |
| ISBN号 | 978-1-61284-775-7 |
| 源URL | [http://ir.sia.cn/handle/173321/8736]  |
| 专题 | 沈阳自动化研究所_机器人学研究室 |
| 通讯作者 | Liu LQ(刘连庆); Wang YC(王越超) |
| 作者单位 | 1.Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences, Beijing, 100071, China 2.State Key Laboratory of Robotics, Shenyang Institute of Automation Chinese Academy of Sciences, Shenyang, 110016, China 3.Graduate University of Chinese Academy of Sciences, Beijing, 100049, China 4.Department of Electrical and Computer Engineering, Michigan State University, East Lansing, MI 48824, United States 5.Department of Electrical and Computer Engineering, University of Pittsburgh, Pittsburgh, PA,15261, United States 6.Department of Micro Engineering, Kyoto University, Kyoto, 606-8501, Japan 7.State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica Chinese Academy of Sciences, Shanghai, 201203, China |
| 推荐引用方式 GB/T 7714 | Li M,Liu LQ,Wang YC,et al. Imaging and measuring the protein distribution of lymphoma cells using atomic force microscopy[C]. 见:. Kaohsiung, Taiwan. February 20-23, 2011. |
入库方式： OAI收割
来源：沈阳自动化研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。