Investigating the Relationship between CD20-Rituximab Binding Force and Mechanical Properties of Lymphom Cells using Atomic Force Microscopy
文献类型：会议论文
| 作者 | Li M(李密) ; Liu LQ(刘连庆); Wang YC(王越超); Dong ZL(董再励); Xiao XB(肖秀斌); Zhang WJ(张伟京)
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| 出版日期 | 2012 |
| 会议日期 | August 29 - September1, 2012 |
| 会议地点 | Xi'an, China |
| 关键词 | Atomic Force Microscopy Lymphoma Rituximab |
| 页码 | 50-53 |
| 英文摘要 | The invention of atomic force microscopy (AFM) provides new technology for investigating the physiological activities at the single cell and single molecule levels. Lymphoma Raji cells were adsorbed onto the glass slides by coating the glass slides with poly-L-lysine. Rituximabs (anti-CD20 antibody) were linked onto the AFM tip by PEG linker and the CD20-Rituximab binding forces were measured on three lymphoma Raji cells. Then the mechanical properties of the Raji cells were dynamically measured after the Rituximab activation by obtaining force curves on the cell surface. The mechanical properties of lymphoma cells kept stable at the first 20-30 min, and then decreased markedly. Besides, as the CD20-Rituximab binding force increased, the mechanical properties decrease rate increased. The experimental results indicated that the Rituximab’s effect is related to CD20-Rituximab binding force and can improve our understanding of Rituximab’s variable efficacies in different patients. |
| 产权排序 | 1 |
| 会议录 | 2012 International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3M-NANO)
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| 会议录出版者 | IEEE |
| 会议录出版地 | New York, USA |
| 语种 | 英语 |
| ISBN号 | 978-1-4673-4590-3 |
| WOS记录号 | WOS:000319774400010 |
| 源URL | [http://ir.sia.cn/handle/173321/10203]  |
| 专题 | 沈阳自动化研究所_机器人学研究室 |
| 通讯作者 | Liu LQ(刘连庆); Wang YC(王越超) |
| 作者单位 | 1.Department of Lymphoma, Affiliated Hospital of Military Medical Academy of Sciences, Beijing, China 2.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China 3.Graduate University of Chinese Academy of Sciences, Beijing, China 4.Department of Electrical and Computer Engineering, Michigan State University, East Lansing, MI, United States 5.State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China |
| 推荐引用方式 GB/T 7714 | Li M,Liu LQ,Wang YC,et al. Investigating the Relationship between CD20-Rituximab Binding Force and Mechanical Properties of Lymphom Cells using Atomic Force Microscopy[C]. 见:. Xi'an, China. August 29 - September1, 2012. |
入库方式： OAI收割
来源：沈阳自动化研究所
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