Research progress in quantifying the mechanical properties of single living cells using atomic force microscopy
文献类型:期刊论文
作者 | Li M(李密); Liu LQ(刘连庆); Wang YC(王越超) |
刊名 | CHINESE SCIENCE BULLETIN |
出版日期 | 2014 |
卷号 | 59期号:31页码:4020-4029 |
ISSN号 | 1001-6538 |
关键词 | Atomic Force Microscopy Cellular Mechanical Properties Indentation Force Curve Elastic Modulus |
产权排序 | 1 |
英文摘要 | The advent of atomic force microscopy (AFM) provides a powerful tool for investigating the behaviors of single living cells under near physiological conditions. Besides acquiring the images of cellular ultra-microstructures with nanometer resolution, the most remarkable advances are achieved on the use of AFM indenting technique to quantify the mechanical properties of single living cells. By indenting single living cells with AFM tip, we can obtain the mechanical properties of cells and monitor their dynamic changes during the biological processes (e.g., after the stimulation of drugs). AFM indentation-based mechanical analysis of single cells provides a novel approach to characterize the behaviors of cells from the perspective of biomechanics, considerably complementing the traditional biological experimental methods. Now, AFM indentation technique has been widely used in the life sciences, yielding a large amount of novel information that is meaningful to our understanding of the underlying mechanisms that govern the cellular biological functions. Here, based on the authors’ own researches on AFM measurement of cellular mechanical properties, the principle and method of AFM indentation technique was presented, the recent progress of measuring the cellular mechanical properties using AFM was summarized, and the challenges of AFM single-cell nanomechanical analysis were discussed. |
WOS关键词 | CANCER-CELLS ; TUMOR PROGRESSION ; AFM-CANTILEVERS ; ELASTIC-MODULUS ; STIFFNESS ; DYNAMICS ; CYTOSKELETON ; SURFACE ; SHAPE ; DEFORMABILITY |
WOS研究方向 | Science & Technology - Other Topics |
语种 | 英语 |
WOS记录号 | WOS:000342421100011 |
资助机构 | National Natural Science Foundation of China [61175103, 61327014] ; CAS FEA International Partnership Program for Creative Research Teams |
公开日期 | 2014-11-03 |
源URL | [http://ir.sia.cn/handle/173321/15264] |
专题 | 沈阳自动化研究所_机器人学研究室 |
通讯作者 | Liu LQ(刘连庆); Wang YC(王越超) |
作者单位 | 1.University of Chinese Academy of Sciences, Beijing, China 2.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China 3.Department of Mechanical and Biomedical EngineeringCity University of Hong Kong, Hong Kong, China |
推荐引用方式 GB/T 7714 | Li M,Liu LQ,Wang YC. Research progress in quantifying the mechanical properties of single living cells using atomic force microscopy[J]. CHINESE SCIENCE BULLETIN,2014,59(31):4020-4029. |
APA | Li M,Liu LQ,&Wang YC.(2014).Research progress in quantifying the mechanical properties of single living cells using atomic force microscopy.CHINESE SCIENCE BULLETIN,59(31),4020-4029. |
MLA | Li M,et al."Research progress in quantifying the mechanical properties of single living cells using atomic force microscopy".CHINESE SCIENCE BULLETIN 59.31(2014):4020-4029. |
入库方式: OAI收割
来源:沈阳自动化研究所
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