Valve-based consecutive bioprinting method for multimaterial tissue-like constructs with controllable interfaces
文献类型:期刊论文
作者 | Wang HR(王赫然)2,3,4![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() |
刊名 | BIOFABRICATION
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出版日期 | 2021 |
卷号 | 13期号:3页码:1-12 |
关键词 | bioprinting multimaterial construct material interface printing efficiency vascularized tissue |
ISSN号 | 1758-5082 |
产权排序 | 1 |
英文摘要 | Bioprinting is a promising technology focusing on tissue manufacturing, whose vital problem is the precise assembly of multiple materials. As the primary solution, the extrusion-based multi-printhead bioprinting (MPB) method requires printhead switching during the printing process, which induces inefficient motion time and material interface defects. We present a valve-based consecutive bioprinting (VCB) method to resolve these problems, containing a precise integrated switching printhead and a well-matched voxelated digital model. The rotary valve built-in the VCB printhead guarantees the precise assembling of different materials at the interface isolated from the viscoelastic inks' elastic potential energy in the cartridge. We study the coordinated control approach of the valve rotation and pressure adjustment to achieve the seamless switching, leading to a controllable multimaterial interface, including boundary and suture structure. Furthermore, we compare the VCB method and MPB method, quantitatively and comprehensively, indicating that the VCB method obtained greater mechanical strength (maximum tensile deformation increased by 44.37%) and higher printing efficiency (effective time ratio increased by 29.48%). As an exemplar, we fabricate a muscle-like tissue with a vascular tree, suture interface encapsulating C2C12, and human dermal fibroblasts (HDFB) cells, then placed it in complete medium with continuous perfusion for 5 d. Our study suggests that the VCB method is sufficient to fabricate heterogeneous tissues with complex multimaterial interfaces. |
资助项目 | National Key Research and Development Project[2020YFB1313100] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA16020803] ; National Natural Science Foundation of China[51875557] ; Research Equipment Development Program of the Chinese Academy of Sciences[YZ201545] ; Research Equipment Development Program of the Chinese Academy of Sciences[YJKYYQ20170042] ; Research Equipment Development Program of the Chinese Academy of Sciences[YJKYYQ20190045] ; National High Technology Research and Development Program of China (863 Program)[2015AA020312] ; National Key Research and Development Program of China[2017YFC1104900] ; Foundation of State Key Laboratory of Robotics[2017-Z16] |
WOS研究方向 | Engineering ; Materials Science |
语种 | 英语 |
WOS记录号 | WOS:000639507900001 |
资助机构 | National Key Research and Development Project [2020YFB1313100] ; Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDA16020803] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [51875557] ; Research Equipment Development Program of the Chinese Academy of Sciences [YZ201545, YJKYYQ20170042, YJKYYQ20190045] ; National High Technology Research and Development Program of China (863 Program)National High Technology Research and Development Program of China [2015AA020312] ; National Key Research and Development Program of China [2017YFC1104900] ; Foundation of State Key Laboratory of Robotics [2017-Z16] |
源URL | [http://ir.sia.cn/handle/173321/28736] ![]() |
专题 | 沈阳自动化研究所_机器人学研究室 |
通讯作者 | Zheng XF(郑雄飞) |
作者单位 | 1.State Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China 2.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China 3.Institutes for Robotics and Intelligent Manufacturing, Chinese Academy of Sciences, Shenyang 110169, China 4.University of Chinese Academy of Sciences, Beijing 100049, China |
推荐引用方式 GB/T 7714 | Wang HR,Guo K,Zhang LM,et al. Valve-based consecutive bioprinting method for multimaterial tissue-like constructs with controllable interfaces[J]. BIOFABRICATION,2021,13(3):1-12. |
APA | Wang HR.,Guo K.,Zhang LM.,Zhu HX.,Li SJ.,...&Zheng XF.(2021).Valve-based consecutive bioprinting method for multimaterial tissue-like constructs with controllable interfaces.BIOFABRICATION,13(3),1-12. |
MLA | Wang HR,et al."Valve-based consecutive bioprinting method for multimaterial tissue-like constructs with controllable interfaces".BIOFABRICATION 13.3(2021):1-12. |
入库方式: OAI收割
来源:沈阳自动化研究所
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