Large full-thickness wounded skin regeneration using 3D-printed elastic scaffold with minimal functional unit of skin
文献类型:期刊论文
作者 | Chang P(常鹏)1; Li SJ(李世杰)2; Sun, Qian4; Guo K(郭凯)2; Wang HR(王赫然)2; Li S(李松)2; Zhang LM(张黎明)2; Xie YB(解勇宝)2; Zheng XF(郑雄飞)2; Liu YH(刘云辉)1,3 |
刊名 | JOURNAL OF TISSUE ENGINEERING |
出版日期 | 2022 |
卷号 | 13页码:1-17 |
ISSN号 | 2041-7314 |
关键词 | 3D-printed PLCL scaffold tissue engineering skin skin wound healing minimal functional unit of skin biomaterial scaffolds |
产权排序 | 2 |
英文摘要 | Traditional tissue engineering skin are composed of living cells and natural or synthetic scaffold. Besize the time delay and the risk of contamination involved with cell culture, the lack of autologous cell source and the persistence of allogeneic cells in heterologous grafts have limited its application. This study shows a novel tissue engineering functional skin by carrying minimal functional unit of skin (MFUS) in 3D-printed polylactide-co-caprolactone (PLCL) scaffold and collagen gel (PLCL + Col + MFUS). MFUS is full-layer micro skin harvested from rat autologous tail skin. 3D-printed PLCL elastic scaffold has the similar mechanical properties with rat skin which provides a suitable environment for MFUS growing and enhances the skin wound healing. Four large full-thickness skin defects with 30 mm diameter of each wound are created in rat dorsal skin, and treated either with tissue engineering functional skin (PLCL + Col + MFUS), or with 3D-printed PLCL scaffold and collagen gel (PLCL + Col), or with micro skin islands only (Micro skin), or without treatment (Normal healing). The wound treated with PLCL + Col + MFUS heales much faster than the other three groups as evidenced by the fibroblasts migration from fascia to the gap between the MFUS dermis layer, and functional skin with hair follicles and sebaceous gland has been regenerated. The PLCL + Col treated wound heals faster than normal healing wound, but no skin appendages formed in PLCL + Col-treated wound. The wound treated with micro skin islands heals slower than the wounds treated either with tissue engineering skin (PLCL + Col + MFUS) or with PLCL + Col gel. Our results provide a new strategy to use autologous MFUS instead seed cells as the bio-resource of engineering skin for large full-thickness skin wound healing. |
语种 | 英语 |
WOS记录号 | WOS:000740691400001 |
资助机构 | Shenyang Science and Technology Bureau [19-109-4-02, 21-172-9-15] ; Guangdong Climbing Peaks Program for high-level hospital development [202000240] ; science&Technology Department of Liaoning Province [2021-KF-22-16] ; state Key Laboratory of Robotics, China [2021-KF-22-16] ; Shengjing Hospital 345 Talent Project |
源URL | [http://ir.sia.cn/handle/173321/30258] |
专题 | 沈阳自动化研究所_机器人学研究室 |
通讯作者 | Zheng XF(郑雄飞); Liu YH(刘云辉) |
作者单位 | 1.Department of Neurosurgery and Plastic and Reconstructive Surgery, Shengjing Hospital of China Medical University, Shenyang, China 2.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang, China 3.Liaoning Medical Surgery and Rehabilitation Robot Engineering Research Center, Shenyang, China 4.Experimental Animal Center, General Hospital of Northern Center Command, Shenyang, China |
推荐引用方式 GB/T 7714 | Chang P,Li SJ,Sun, Qian,et al. Large full-thickness wounded skin regeneration using 3D-printed elastic scaffold with minimal functional unit of skin[J]. JOURNAL OF TISSUE ENGINEERING,2022,13:1-17. |
APA | Chang P.,Li SJ.,Sun, Qian.,Guo K.,Wang HR.,...&Liu YH.(2022).Large full-thickness wounded skin regeneration using 3D-printed elastic scaffold with minimal functional unit of skin.JOURNAL OF TISSUE ENGINEERING,13,1-17. |
MLA | Chang P,et al."Large full-thickness wounded skin regeneration using 3D-printed elastic scaffold with minimal functional unit of skin".JOURNAL OF TISSUE ENGINEERING 13(2022):1-17. |
入库方式: OAI收割
来源:沈阳自动化研究所
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