Characterization of interconnectivity of gelatin methacrylate hydrogels using photoacoustic imaging
文献类型:期刊论文
作者 | Zhao WX(赵文秀)1,2,3; Yu HB(于海波)1,2; Ge ZX(葛治星)1,2,3; Wang XD(王晓朵)1,2; Zhang, YZ(章昱昭)1,2,3; Wen YD(文扬东)4; Luo H(罗昊)1,2,3; Liu LQ(刘连庆)1,2; Li WJ(李文荣)1,2,5 |
刊名 | LAB ON A CHIP |
出版日期 | 2022 |
卷号 | 22期号:4页码:727-732 |
ISSN号 | 1473-0197 |
产权排序 | 1 |
英文摘要 | Hydrogels can provide a three-dimensional microenvironment for cells and thus serve as an extracellular matrix in a biofabrication process. The properties of hydrogels, such as their porosity and mechanical properties, significantly influence the cell growth. However, there is still a lack of effective methods for characterizing the hydrogel structure noninvasively. Herein, a photoacoustic (PA) imaging-based method is proposed for the characterization of gelatin methacrylate (GelMA) hydrogels. Owing to their high PA contrast, red blood cells (RBCs) are included as mediators in the GelMA hydrogel to analyze its pore distribution. The interconnectivity of the pores is further analyzed through the lysis of RBCs. The diffusion of the RBC lysis buffer in the GelMA is consistent with the trend observed in simulations. The analyzed vitality of HEK293 cells in different GelMA hydrogels reveals that understanding the diffusion of solutes (i.e., nutrients) is a potential strategy to optimize the hydrogel parameters during biofabrication. |
WOS关键词 | STEM-CELLS ; 3D |
资助项目 | National Key R&D Program of China[2018YFB1304900] ; National Natural Science Foundation of China[61925307] ; National Natural Science Foundation of China[61727811] ; National Natural Science Foundation of China[61973298] ; National Natural Science Foundation of China[61803366] ; CAS Interdisciplinary Innovation Team[JCTD-2019-09] ; Hong Kong Research Grants Council[11213817] ; CAS-RGC Joint Laboratory Funding Scheme[JLFS/E-104/18] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences[Y201943] |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics ; Instruments & Instrumentation |
语种 | 英语 |
WOS记录号 | WOS:000742162100001 |
资助机构 | National Key R&D Program of China [2018YFB1304900] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [61925307, 61727811, 61973298, 61803366] ; CAS Interdisciplinary Innovation Team [JCTD-2019-09] ; Hong Kong Research Grants CouncilHong Kong Research Grants Council [11213817] ; CAS-RGC Joint Laboratory Funding Scheme [JLFS/E-104/18] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences [Y201943] |
源URL | [http://ir.sia.cn/handle/173321/30260] |
专题 | 沈阳自动化研究所_机器人学研究室 |
通讯作者 | Yu HB(于海波); Li WJ(李文荣) |
作者单位 | 1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China 2.Institutes for Robotics and Intelligent Manufacturing, Chinese Academy of Sciences, Shenyang 110016, China 3.University of Chinese Academy of Sciences, Beijing 100049, China 4.Institute of Urban Rail Transportation, Southwest Jiaotong University, Chengdu 610000, China 5.Department of Mechanical Engineering, City University of Hong Kong, Hong Kong SAR, China |
推荐引用方式 GB/T 7714 | Zhao WX,Yu HB,Ge ZX,et al. Characterization of interconnectivity of gelatin methacrylate hydrogels using photoacoustic imaging[J]. LAB ON A CHIP,2022,22(4):727-732. |
APA | Zhao WX.,Yu HB.,Ge ZX.,Wang XD.,Zhang, YZ.,...&Li WJ.(2022).Characterization of interconnectivity of gelatin methacrylate hydrogels using photoacoustic imaging.LAB ON A CHIP,22(4),727-732. |
MLA | Zhao WX,et al."Characterization of interconnectivity of gelatin methacrylate hydrogels using photoacoustic imaging".LAB ON A CHIP 22.4(2022):727-732. |
入库方式: OAI收割
来源:沈阳自动化研究所
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