Isolation method of Saccharomyces cerevisiae from red blood cells based on the optically induced dielectrophoresis technique for the rapid detection of fungal infections
文献类型:期刊论文
作者 | Du, Mingao2; Liu F(刘飞)2; Luan XL(栾小丽)2; Li GX(李恭新)1,2 |
刊名 | Biomedical Optics Express |
出版日期 | 2022 |
卷号 | 13期号:2页码:559-570 |
ISSN号 | 2156-7085 |
产权排序 | 2 |
英文摘要 | Saccharomyces cerevisiae (S. cerevisiae) has been classically used to treat diarrhea and diarrhea-related diseases. However, in the past two decades, fungal infections caused by S. cerevisiae have been increasing among immunocompromised patients, and it takes too long to isolate S. cerevisiae from blood to diagnose it in time. In this paper, a new method for the isolation and selection of S. cerevisiae from red blood cells (RBC) is proposed by designing a microfluidic chip with an optically-induced dielectrophoresis (ODEP) system. It was verified by theory and experiments that the magnitude and direction of the dielectrophoresis force applied on RBCs and S. cerevisiae are different, which determine that the S. cerevisiae can be isolated from RBCs by the ODEP system. By designing the specific light images and the dynamic separation mode, the optimal operating conditions were experimentally achieved for acquiring higher purity of S. cerevisiae. The purity ranges were up to 95.9%-97.3%. This work demonstrates a promising tool for efficient and effective purification of S. cerevisiae from RBCs and provides a novel method of S. cerevisiae isolation for the timely diagnosis of fungal infections. |
WOS关键词 | FUNGEMIA ; MICROPARTICLES ; MANIPULATION ; CAPACITANCE ; PARTICLE ; CTCS |
资助项目 | National Natural Science Foundation of China[61833007] ; National Natural Science Foundation of China[61903157] ; Joint Open Fund of the State Key Laboratory of Robotics[2021-KF-22-04] |
WOS研究方向 | Biochemistry & Molecular Biology ; Optics ; Radiology, Nuclear Medicine & Medical Imaging |
语种 | 英语 |
WOS记录号 | WOS:000750864600004 |
资助机构 | National Natural Science Foundation of China (61833007, 61903157) ; Joint Open Fund of the State Key Laboratory of Robotics (2021-KF-22-04) |
源URL | [http://ir.sia.cn/handle/173321/30330] |
专题 | 沈阳自动化研究所_机器人学研究室 |
通讯作者 | Li GX(李恭新) |
作者单位 | 1.State Key Laboratory of Robotics, Shenyang Institute of Automation, Chinese Academy of Sciences, Shenyang 110016, China 2.Key Laboratory of Advanced Process Control for Light Industry, Ministry of Education, Institute of Automation, Jiangnan University, Wuxi 214122, China |
推荐引用方式 GB/T 7714 | Du, Mingao,Liu F,Luan XL,et al. Isolation method of Saccharomyces cerevisiae from red blood cells based on the optically induced dielectrophoresis technique for the rapid detection of fungal infections[J]. Biomedical Optics Express,2022,13(2):559-570. |
APA | Du, Mingao,Liu F,Luan XL,&Li GX.(2022).Isolation method of Saccharomyces cerevisiae from red blood cells based on the optically induced dielectrophoresis technique for the rapid detection of fungal infections.Biomedical Optics Express,13(2),559-570. |
MLA | Du, Mingao,et al."Isolation method of Saccharomyces cerevisiae from red blood cells based on the optically induced dielectrophoresis technique for the rapid detection of fungal infections".Biomedical Optics Express 13.2(2022):559-570. |
入库方式: OAI收割
来源:沈阳自动化研究所
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