This study developed a new cross-platform instrument for microstructure turbulence measurement (CPMTM) and evaluated its performance. The CPMTM is designed as an “all-in-one” payload that can be easily integrated with a variety of marine instrumentation platforms. The sen-sors in the CPMTM include two shear probes, a fast-response temperature probe, and an accelerom-eter for monitoring vibrations. In addition, a custom-designed flexible connection vibration-damp-ing device is used to isolate platform vibrations. To validate the CPMTM performance, a direct comparison was carried out with a reference acoustic Doppler velocimeter in a controlled flume for four background turbulence levels. The results of the comparison show that the velocity spectra measured by the CPMTM and ADV w components are in agreement, which demonstrates the ability of the CPMTM to acquire accurate turbulence data. Furthermore, the CPMTM was integrated into the long-range Sea-Whale 2000 AUV and tested in the northern South China Sea in September 2020. The data collected by the CPMTM show that the measured shear spectrum of the noise reduction agrees well with the empirical Nasmyth spectrum. Turbulent kinetic energy dissipation rates as low as 7 × 10−10 W kg−1 can be resolved. Laboratory and field experiments illustrate that the CPMTM has an extraordinarily low noise level and is validated for turbulence measurements.