Shabyar Ameliorates High Glucose Induced Retinal Pigment Epithelium Injury Through Suppressing Aldose Reductase and AMPK/mTOR/ULK1 Autophagy Pathway
文献类型:期刊论文
作者 | Liu, XY (Liu, Xiao Yan) [1] , [2] , [3]; Peng, J (Peng, Jun) [1] , [2]; He, F (He, Fei) [1]; Tursun, X (Tursun, Xirali) [4]; Li, SP (Li, Shu Ping) [1]; Xin, XL (Xin, Xue Lei) [1]; Aisa, HA (Aisa, Haji Akber) [1] |
刊名 | FRONTIERS IN PHARMACOLOGY |
出版日期 | 2022 |
卷号 | 13期号:5页码:1-15 |
ISSN号 | 1663-9812 |
关键词 | shabyar retinal pigment epithelium autophagy aldose reductase traditional medicine formula |
DOI | 10.3389/fphar.2022.852945 |
英文摘要 | Shabyar (SBA) is a traditional medicine formula for relieving vision loss caused by factors including diabetic retinopathy (DR) in clinics. However, the mechanism of it on retina protective effect still unclear. The present study aimed to investigate whether its protective effect was related to aldose reductase (AR) inhibition and retinal pigment epithelial cell injury mediated by autophagy or not. Human retinal pigment epithelial cells (ARPE-19) induced by high glucose was used as a model in vitro, with Epalrestat (EPL, AR inhibitor) and Difrarel (DFR, DR therapeutic drug) as positive controls. Western blotting and Polyol pathway products assay showed that SBA reduced the expression of AR protein and the content of ROS, and sorbitol, increased the level of Na+-K+-ATPase and alleviated cell edema. Western blotting and DCFH-DA probe assay showed that SBA decreased pAMPK/AMPK and pULK1/ULK1 which associated with autophagy initiation, down-regulated Beclin-1, Atg3, Atg5, Atg7, LC3 II and Bax/Bcl2 ratio, and up-regulated pmTOR/mTOR, SQSTM1/p62 and mitochondrial membrane potential (MMP), reduces intracellular autophagosomes. Real-Time PCR assay showed that SBA had no significant effect on mRNA expression of AR and mTOR. These data demonstrated that SBA treatment inhibits the autophagy of ARPE-19 through the AMPK/mTOR/ULK1 signaling pathway, and reduced early-stage apoptosis occurred by high glucose. These findings reveal the protective role and mechanism of SBA on retinal pigment epithelium, and provide experimental basis for the clinical application of SBA in the treatment of DR. |
WOS记录号 | WOS:000802259400001 |
源URL | [http://ir.xjipc.cas.cn/handle/365002/8382] |
专题 | 新疆理化技术研究所_省部共建新疆特有药用资源利用重点实验室 新疆理化技术研究所_资源化学研究室 |
通讯作者 | Xin, XL (Xin, Xue Lei) [1]; Aisa, HA (Aisa, Haji Akber) [1] |
作者单位 | 1.Inst Xinjiang Tradit Uyghur Med, Urumqi, Peoples R China 2.Xinjiang Inst Mat Med, Urumqi, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, State Key Lab Basis Xinjiang Indigenous Med Plant, Urumqi, Peoples R China |
推荐引用方式 GB/T 7714 | Liu, XY ,Peng, J ,He, F ,et al. Shabyar Ameliorates High Glucose Induced Retinal Pigment Epithelium Injury Through Suppressing Aldose Reductase and AMPK/mTOR/ULK1 Autophagy Pathway[J]. FRONTIERS IN PHARMACOLOGY,2022,13(5):1-15. |
APA | Liu, XY .,Peng, J .,He, F .,Tursun, X .,Li, SP .,...&Aisa, HA .(2022).Shabyar Ameliorates High Glucose Induced Retinal Pigment Epithelium Injury Through Suppressing Aldose Reductase and AMPK/mTOR/ULK1 Autophagy Pathway.FRONTIERS IN PHARMACOLOGY,13(5),1-15. |
MLA | Liu, XY ,et al."Shabyar Ameliorates High Glucose Induced Retinal Pigment Epithelium Injury Through Suppressing Aldose Reductase and AMPK/mTOR/ULK1 Autophagy Pathway".FRONTIERS IN PHARMACOLOGY 13.5(2022):1-15. |
入库方式: OAI收割
来源:新疆理化技术研究所
其他版本
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。