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The Non-canonical Tetratricopeptide Repeat (TPR) Domain of Fluorescent (FLU) Mediates Complex Formation with Glutamyl-tRNA Reductase

文献类型：期刊论文

作者Zhang, Min; Zhang, Feilong; Fang, Ying2; Chen, Xuemin2; Chen, Yuhong; Zhang, Wenxia; Dai, Huai-En; Lin, Rongcheng1; Liu, Lin1
刊名JOURNAL OF BIOLOGICAL CHEMISTRY
出版日期2015
卷号290期号:28页码:17559-17565
ISSN号0021-9258
DOI10.1074/jbc.M115.662981
文献子类Article
英文摘要The tetratricopeptide repeat (TPR)-containing protein FLU is a negative regulator of chlorophyll biosynthesis in plants. It directly interacts through its TPR domain with glutamyl-tRNA reductase (GluTR), the rate-limiting enzyme in the formation of delta-aminolevulinic acid (ALA). Delineation of how FLU binds to GluTR is important for understanding the molecular basis for FLU-mediated repression of synthesis of ALA, the universal tetrapyrrole precursor. Here, we characterize the FLU-GluTR interaction by solving the crystal structures of the uncomplexed TPR domain of FLU (FLUTPR) at 1.45-angstrom resolution and the complex of the dimeric domain of GluTR bound to FLUTPR at 2.4-angstrom resolution. Three non-canonical TPR motifs of each FLUTPR form a concave surface and clamp the helix bundle in the C-terminal dimeric domain of GluTR. We demonstrate that a 2: 2 FLUTPR-GluTR complex is the functional unit for FLU-mediated GluTR regulation and suggest that the formation of the FLU-GluTR complex prevents glutamyl-tRNA, the GluTR substrate, from binding with this enzyme. These results also provide insights into the spatial regulation of ALA synthesis by the membrane-located FLU protein.
学科主题Biochemistry & Molecular Biology
出版地BETHESDA
电子版国际标准刊号1083-351X
WOS关键词5-AMINOLEVULINIC ACID SYNTHESIS ; TETRAPYRROLE BIOSYNTHESIS ; SINGLET OXYGEN ; CHLOROPHYLL BIOSYNTHESIS ; ARABIDOPSIS-THALIANA ; NEGATIVE REGULATOR ; PROTEIN ; BARLEY ; PLANTS ; HEME
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000357730900046
出版者AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
资助机构National Natural Science Foundation of China [31370759] ; National Basic Research Program of China [2011CBA00901] ; Hundred Talents Program of the Chinese Academy of Sciences ; [KGZD-EW-T05]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/25664]  
专题中科院光生物学重点实验室
作者单位1.Anhui Univ, Sch Life Sci, Hefei 230601, Anhui, Peoples R China
2.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Photosynthesis Res Ctr, Beijing 100093, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Min,Zhang, Feilong,Fang, Ying,et al. The Non-canonical Tetratricopeptide Repeat (TPR) Domain of Fluorescent (FLU) Mediates Complex Formation with Glutamyl-tRNA Reductase[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2015,290(28):17559-17565.
APA Zhang, Min.,Zhang, Feilong.,Fang, Ying.,Chen, Xuemin.,Chen, Yuhong.,...&Liu, Lin.(2015).The Non-canonical Tetratricopeptide Repeat (TPR) Domain of Fluorescent (FLU) Mediates Complex Formation with Glutamyl-tRNA Reductase.JOURNAL OF BIOLOGICAL CHEMISTRY,290(28),17559-17565.
MLA Zhang, Min,et al."The Non-canonical Tetratricopeptide Repeat (TPR) Domain of Fluorescent (FLU) Mediates Complex Formation with Glutamyl-tRNA Reductase".JOURNAL OF BIOLOGICAL CHEMISTRY 290.28(2015):17559-17565.

入库方式： OAI收割

来源：植物研究所

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