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Crystal structure of the catalytic subunit of magnesium chelatase

文献类型：期刊论文

作者Chen, Xuemin3; Pu, Hua3; Fang, Ying3; Wang, Xiao3; Zhao, Shun3; Lin, Yajing4; Zhang, Min1; Dai, Huai-En; Gong, Weimin4,5; Liu, Lin
刊名NATURE PLANTS
出版日期2015
卷号1期号:9
ISSN号2055-026X
DOI10.1038/NPLANTS.2015.125
文献子类Article
英文摘要Tetrapyrroles, including haem and chlorophyll, play vital roles for various biological processes, such as respiration and photosynthesis, and their biosynthesis is critical for virtually all organisms. In photosynthetic organisms, magnesium chelatase (MgCh) catalyses insertion of magnesium into the centre of protoporphyrin IX, the branch-point precursor for both haem and chlorophyll, leading tetrapyrrole biosynthesis into the magnesium branch1,2. This reaction needs a cooperated action of the three subunits of MgCh: the catalytic subunit ChlH and two AAA(+) subunits, ChlI and ChlD ( refs 3-5). To date, the mechanism of MgCh awaits further elucidation due to a lack of high-resolution structures, especially for the similar to 150 kDa catalytic subunit. Here we report the crystal structure of ChlH from the photosynthetic cyanobacterium Synechocystis PCC 6803, solved at 2.5 angstrom resolution. The active site is buried deeply inside the protein interior, and the surrounding residues are conserved throughout evolution. This structure helps to explain the loss of function reported for the cch and gun5 mutations of the ChlH subunit, and to provide the molecular basis of substrate channelling during the magnesium-chelating process. The structure advances our understanding of the holoenzyme of MgCh, a metal chelating enzyme other than ferrochelatase.
学科主题Plant Sciences
出版地LONDON
电子版国际标准刊号2055-0278
WOS关键词PROTOPORPHYRINOGEN-IX OXIDASE ; MG-CHELATASE ; H-SUBUNIT ; SYNECHOCYSTIS PCC6803 ; ATPASE ACTIVITY ; BARLEY MUTANTS ; ENZYME ; GUN4 ; BIOSYNTHESIS ; EXPRESSION
WOS研究方向Science Citation Index Expanded (SCI-EXPANDED)
语种英语
WOS记录号WOS:000364383900002
出版者NATURE PUBLISHING GROUP
资助机构National Basic Research Program of China [2011CBA00901] ; Hundred Talents Program of Chinese Academy of Sciences ; [KGZD-EW-T05]
源URL[http://ir.ibcas.ac.cn/handle/2S10CLM1/25941]  
专题中科院光生物学重点实验室
作者单位1.Chinese Acad Sci, Inst Biophys, Lab Noncoding RNA, Beijing 100101, Peoples R China
2.Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230027, Anhui, Peoples R China
3.Chinese Acad Sci, Inst Bot, Key Lab Photobiol, Photosynth Res Ctr, Beijing 100093, Peoples R China
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
5.Anhui Univ, Sch Life Sci, Hefei 230601, Anhui, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Xuemin,Pu, Hua,Fang, Ying,et al. Crystal structure of the catalytic subunit of magnesium chelatase[J]. NATURE PLANTS,2015,1(9).
APA Chen, Xuemin.,Pu, Hua.,Fang, Ying.,Wang, Xiao.,Zhao, Shun.,...&Liu, Lin.(2015).Crystal structure of the catalytic subunit of magnesium chelatase.NATURE PLANTS,1(9).
MLA Chen, Xuemin,et al."Crystal structure of the catalytic subunit of magnesium chelatase".NATURE PLANTS 1.9(2015).

入库方式： OAI收割

来源：植物研究所

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